IHCImmunohistochemistry of paraffin-embedded human testis using A002700(EIF2S2 antibody) at dilution of 1:50
Western Blotmouse liver tissue were subjected to SDS PAGE followed by western blot with A002700(EIF2S2 antibody) at dilution of 1:1000
Recommended dilutionWB : 1:500-1:5000
Product Description specificaleIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.