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Alternative name
DICER, HERNA, KIAA0928 antibody
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Catalog
A002387
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IHC,IF,IP
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PurificationImmunogen affinity purified
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Immunogendicer 1, ribonuclease type III
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IHCImmunohistochemistry of paraffin-embedded human testis tissue slide using A002387( DICER1 Antibody) at dilution of 1:200
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Western BlotK-562 cells were subjected to SDS PAGE followed by western blot with A002387(DICER1 antibody) at dilution of 1:200
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Recommended dilutionWB : 1:200-1:1000
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Product Description specificalDouble-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA- induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
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Gene nameDICER1
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Uniprot IDQ9UPY3
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Observed M.W.220-250 kDa,90 kDa
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