StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
PurificationImmunogen affinity purified
Immunogenabsent in melanoma 2
IHCImmunohistochemistry of paraffin-embedded human testis using A000238(AIM2 antibody) at dilution of 1:100
Western BlotK-562 cells were subjected to SDS PAGE followed by western blot with A000238(AIM2 antibody) at dilution of 1:1000
Recommended dilutionWB :1:500-1:5000;IHC:1:20-200
Product Description specificalInvolved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8 (By similarity). Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.