-
Alternative name
C22orf12, DGCR8, DGCRK6, Gy1 antibody
-
Catalog
A002351
-
Size100μg
-
Formliquid
-
Purity≥95% as determined by SDS-PAGE
-
StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
-
ClonalityPolyclonal Antibody
-
Host Rabbit
-
Species ReactivityHuman,Mouse ,Rat
-
Applications Tested/SuitableELISA,WB,IHC,IF,IP,FC
-
PurificationImmunogen affinity purified
-
ImmunogenDiGeorge syndrome critical region gene 8
-
IHCImmunohistochemistry of paraffin-embedded human breast cancer using A002351(DGCR8 antibody) at dilution of 1:100
-
Western BlotHEK-293 cells were subjected to SDS PAGE followed by western blot with A002351(DGCR8 antibody) at dilution of 1:1000
-
Recommended dilutionWB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:50-1:200
IF : 1:20-1:200
-
Product Description specificalComponent of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding (PubMed:15531877, PubMed:15574589, PubMed:15589161, PubMed:16751099, PubMed:16906129, PubMed:16963499, PubMed:17159994). Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (PubMed:25799998). Involved in the silencing of embryonic stem cell self-renewal (By similarity).
-
Gene nameDGCR8
-
Uniprot IDQ8WYQ5
-
Calculated M.W.120 kDa
-
Observed M.W.120 kDa
-