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Alternative name
Active caspase 3, Apopain, CASP 3, CASP3, Caspase 3, Caspase 3 subunit p17, Cleaved Caspase 3, CPP 32, CPP32, CPP-32, CPP32B, Cysteine protease CPP32, P17, Protein Yama, SREBP cleavage activity 1 antibody
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Catalog
A001290
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman,Mouse ,Rat
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Applications Tested/Suitable ELISA,WB,FC,IHC,IF
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PurificationImmunogen affinity purified
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Immunogencaspase 3, apoptosis-related cysteine peptidase
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IHCImmunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using A001290( Caspase 3 Antibody) at dilution of 1:50
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Western BlotTunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with A001290( Caspase 3 Antibody) at dilution of 1:600
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Recommended dilutionWB:1:200-1:2000;IHC:1:20-1:200;IF:1:20-1:200
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Product Description specificalCaspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize p17 of caspase 3 specifically.
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Gene nameCASP3
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Uniprot IDP42574?
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Calculated M.W.32kDa,observed 17kDa
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Observed M.W.17 kDa
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