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Alternative name
CASP 1, CASP1, Caspase 1, Caspase 1/p20/p10, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, p10, p20, P45 antibody
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Catalog
A001284
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Size100μg
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Formliquid
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Purity≥95% as determined by SDS-PAGE
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StoragePBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
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ClonalityPolyclonal Antibody
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Host Rabbit
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Species ReactivityHuman, Mouse ,Rat
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Applications Tested/SuitableELISA,WB,IF
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PurificationImmunogen affinity purified
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Immunogencaspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
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IHCImmunohistochemistry of paraffin-embedded human spleen tissue slide using A001284( Caspase 1 Antibody) at dilution of 1:200
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Western BlotJurkat cells were subjected to SDS PAGE followed by western blot with A001284(CASP1 antibody) at dilution of 1:1000
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Recommended dilutionWB:1:500-1:5000;IHC:1:100-1:400;IF:1:10-1:100
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Product Description specificalCASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.
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Gene nameCASP1
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Uniprot IDP29466
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Calculated M.W.45kDa
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Observed M.W.45-47 kDa,30 kDa,35 kDa
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