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Human F12 ELISA Kit

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  • Alternative name

    Human Hageman factor ELISA Kit;Human HAF ELISA Kit;Human beta-factor XIIa part 2 ELISA Kit;Human HAE3 ELISA Kit;Human HAEX ELISA Kit;Human coagulation factor XII ELISA Kit;Human beta-factor XIIa part 1 ELISA Kit;Human coagulation factor XIIa heavy chain ELISA Kit;Human coagulation factor XIIa light chain ELISA Kit;
  • Catalog
    E009860
  • species
    Human
  • GeneF12
  • Standard CurveHuman F12 ELISA Kit
  • Other Species Mouse F12 ELISA KitBovine F12 ELISA KitGuinea Pig F12 ELISA KitPorcine F12 ELISA KitRat F12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Coagulation Factor XII (F12). No significant cross-reactivity or interference between Coagulation Factor XII (F12) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.137ng/mL.
  • Intended UseHuman F12 ELISA Kit allows for the in vitro quantitative determination of F12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XII (F12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XII (F12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XII (F12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XII (F12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Coagulation factor XIIa light chain Protein information
  • Uniprot ID FA12_HUMAN
  • Uniprot AC P00748; P78339;
  • UniGene Hs.1321;
  • GeneID 2161
  • KEGG hsa:2161;
  • Human Coagulation factor XIIa light chain Protein SEQUENCE

  • SEQUENCE 615 AA; 67792 MW; F5B861BF635EB480 CRC64;

    MRALLLLGFL LVSLESTLSI PPWEAPKEHK YKAEEHTVVL TVTGEPCHFP

    FQYHRQLYHK CTHKGRPGPQ PWCATTPNFD QDQRWGYCLE PKKVKDHCSK

    HSPCQKGGTC VNMPSGPHCL CPQHLTGNHC QKEKCFEPQL LRFFHKNEIW

    YRTEQAAVAR CQCKGPDAHC QRLASQACRT NPCLHGGRCL EVEGHRLCHC

    PVGYTGAFCD VDTKASCYDG RGLSYRGLAR TTLSGAPCQP WASEATYRNV

    TAEQARNWGL GGHAFCRNPD NDIRPWCFVL NRDRLSWEYC DLAQCQTPTQ

    AAPPTPVSPR LHVPLMPAQP APPKPQPTTR TPPQSQTPGA LPAKREQPPS

    LTRNGPLSCG QRLRKSLSSM TRVVGGLVAL RGAHPYIAAL YWGHSFCAGS

    LIAPCWVLTA AHCLQDRPAP EDLTVVLGQE RRNHSCEPCQ TLAVRSYRLH

    EAFSPVSYQH DLALLRLQED ADGSCALLSP YVQPVCLPSG AARPSETTLC

    QVAGWGHQFE GAEEYASFLQ EAQVPFLSLE RCSAPDVHGS SILPGMLCAG

    FLEGGTDACQ GDSGGPLVCE DQAAERRLTL QGIISWGSGC GDRNKPGVYT

    DVAYYLAWIR EHTVS

  • UCSC uc003mgo.5; human.;


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