Human F11 ELISA Kit

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  • Alternative name

    Human FXI ELISA Kit;Human plasma thromboplastin antecedent ELISA Kit;Human PTA ELISA Kit;Human coagulation factor XI ELISA Kit;

  • Catalog
    E009855
  • species
    Human
  • GeneF11
  • Standard CurveHuman F11 ELISA Kit
  • Other Species Mouse F11 ELISA KitBovine F11 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman F11 ELISA Kit allows for the in vitro quantitative determination of F11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Principle of the Assay: FXI ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FXI. Standards or samples are then added to the microtiter plate wells and FXI if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FXI present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FXI are added to each well to "sandwich" the FXI immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FXI and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXI concentration in each sample is interpolated from this standard curve.
  • Human Coagulation factor XIa light chain Protein information
  • Uniprot ID FA11_HUMAN
  • Uniprot AC P03951; D3DP64; Q4W5C2; Q9Y495;
  • UniGene Hs.1430;
  • GeneID 2160
  • KEGG hsa:2160;
  • Human Coagulation factor XIa light chain Protein SEQUENCE
  • SEQUENCE 625 AA; 70109 MW; 147AFA94B7709E8F CRC64;

    MIFLYQVVHF ILFTSVSGEC VTQLLKDTCF EGGDITTVFT PSAKYCQVVC

    TYHPRCLLFT FTAESPSEDP TRWFTCVLKD SVTETLPRVN RTAAISGYSF

    KQCSHQISAC NKDIYVDLDM KGINYNSSVA KSAQECQERC TDDVHCHFFT

    YATRQFPSLE HRNICLLKHT QTGTPTRITK LDKVVSGFSL KSCALSNLAC

    IRDIFPNTVF ADSNIDSVMA PDAFVCGRIC THHPGCLFFT FFSQEWPKES

    QRNLCLLKTS ESGLPSTRIK KSKALSGFSL QSCRHSIPVF CHSSFYHDTD

    FLGEELDIVA AKSHEACQKL CTNAVRCQFF TYTPAQASCN EGKGKCYLKL

    SSNGSPTKIL HGRGGISGYT LRLCKMDNEC TTKIKPRIVG GTASVRGEWP

    WQVTLHTTSP TQRHLCGGSI IGNQWILTAA HCFYGVESPK ILRVYSGILN

    QSEIKEDTSF FGVQEIIIHD QYKMAESGYD IALLKLETTV NYTDSQRPIC

    LPSKGDRNVI YTDCWVTGWG YRKLRDKIQN TLQKAKIPLV TNEECQKRYR

    GHKITHKMIC AGYREGGKDA CKGDSGGPLS CKHNEVWHLV GITSWGEGCA

    QRERPGVYTN VVEYVDWILE KTQAV

  • UCSC uc003iza.2; human. [P03951-1];


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