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Human F9 ELISA Kit

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  • Alternative name

    Human Christmas factor ELISA Kit;Human plasma thromboplastin component ELISA Kit;Human PTC ELISA Kit;Human F9 p22 ELISA Kit;Human FIX ELISA Kit;Human HEMB ELISA Kit;Human P19 ELISA Kit;Human THPH8 ELISA Kit;Human coagulation factor IX ELISA Kit;Human factor 9 ELISA Kit;Human factor IX F9 ELISA Kit;Human plasma thromboplastic component ELISA Kit;
  • Catalog
    E009815
  • species
    Human
  • GeneF9
  • Standard CurveHuman F9 ELISA Kit
  • Other Species Mouse F9 ELISA KitBovine F9 ELISA KitCanine F9 ELISA KitGuinea Pig F9 ELISA KitChicken F9 ELISA KitPorcine F9 ELISA KitRabbit F9 ELISA KitRat F9 ELISA KitSheep F9 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human F9. No significant cross-reactivity or interference between Human F9 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.078 ng/ml
  • Intended UseHuman F9 ELISA Kit allows for the in vitro quantitative determination of F9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FIX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FIX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FIX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FIX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Coagulation factor IXa heavy chain Protein information
  • Uniprot ID FA9_HUMAN
  • Uniprot AC P00740; A8K9N4; F2RM36; Q5FBE1; Q5JYJ8;
  • UniGene Hs.522798;
  • GeneID 2158
  • KEGG hsa:2158;
  • Human Coagulation factor IXa heavy chain Protein SEQUENCE

  • SEQUENCE 461 AA; 51778 MW; C4720C1234477EF5 CRC64;

    MQRVNMIMAE SPGLITICLL GYLLSAECTV FLDHENANKI LNRPKRYNSG

    KLEEFVQGNL ERECMEEKCS FEEAREVFEN TERTTEFWKQ YVDGDQCESN

    PCLNGGSCKD DINSYECWCP FGFEGKNCEL DVTCNIKNGR CEQFCKNSAD

    NKVVCSCTEG YRLAENQKSC EPAVPFPCGR VSVSQTSKLT RAETVFPDVD

    YVNSTEAETI LDNITQSTQS FNDFTRVVGG EDAKPGQFPW QVVLNGKVDA

    FCGGSIVNEK WIVTAAHCVE TGVKITVVAG EHNIEETEHT EQKRNVIRII

    PHHNYNAAIN KYNHDIALLE LDEPLVLNSY VTPICIADKE YTNIFLKFGS

    GYVSGWGRVF HKGRSALVLQ YLRVPLVDRA TCLRSTKFTI YNNMFCAGFH

    EGGRDSCQGD SGGPHVTEVE GTSFLTGIIS WGEECAMKGK YGIYTKVSRY

    VNWIKEKTKL T

  • UCSC uc004fas.2; human. [P00740-1];


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