Coagulation factor V ELISA KIT; Activated protein C cofactor ELISA KIT; Proaccelerin, labile factorCleaved into the following 2 chains:Coagulation factor V heavy chain ELISA KIT; Coagulation factor V light chainF5 ELISA KIT;
Intended UseHuman F5 Ag ELISA Kit allows for the in vitro quantitative determination of F5 Ag , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCardiovascular
Product Description specificalPrinciple of the Assay: F5-Ag ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F5-Ag antibody and an F5-Ag-HRP conjugate. The assay sample and buffer are incubated together with F5-Ag-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F5-Ag concentration since F5-Ag from samples and F5-Ag-HRP conjugate compete for the anti-F5-Ag antibody binding site. Since the number of sites is limited, as more sites are occupied by F5-Ag from the sample, fewer sites are left to bind F5-Ag-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F5-Ag concentration in each sample is interpolated from this standard curve.
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