Human ARL13B ELISA Kit

Alternative name
Human ADP-ribosylation factor-like protein 2-like 1 ELISA Kit;Human ARL2-like protein 1 ELISA Kit;Human ARL2L1 ELISA Kit;Human JBTS8 ELISA Kit;Human ADP ribosylation factor like GTPase 13B ELISA Kit;Human ADP-ribosylation factor-like protein 13B ELISA Kit;Human ADP-ribosylation factor-like 13B ELISA Kit;Human ADP-ribosylation factor-like 2-like 1 ELISA Kit;
Catalog
E000974
species
Human
GeneARL13B
Standard Curve
Other Species Mouse Arl13b ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ARL13B. No significant cross-reactivity or interference between ARL13B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARL13B and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ARL13B ELISA Kit allows for the in vitro quantitative determination of ARL13B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This ARL13B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARL13B. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ARL13B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARL13B antibody and an ARL13B-HRP conjugate. The assay sample and buffer are incubated together with ARL13B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARL13B concentration since ARL13B from samples and ARL13B-HRP conjugate compete for the anti-ARL13B antibody binding site. Since the number of sites is limited, as more sites are occupied by ARL13B from the sample, fewer sites are left to bind ARL13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARL13B concentration in each sample is interpolated from this standard curve.
Human ADP-ribosylation factor-like protein 13B Protein information
Uniprot ID AR13B_HUMAN
Uniprot AC Q3SXY8; D3DN29; G3V1S8; Q504W8; Q8TCL5;
UniGene Hs.533086;
GeneID 200894
KEGG hsa:200894;
Human ADP-ribosylation factor-like protein 13B Protein SEQUENCE
SEQUENCE 428 AA; 48643 MW; 002B38A38D1F7BDD CRC64;
MFSLMASCCG WFKRWREPVR KVTLLMVGLD NAGKTATAKG IQGEYPEDVA
PTVGFSKINL RQGKFEVTIF DLGGGIRIRG IWKNYYAESY GVIFVVDSSD
EERMEETKEA MSEMLRHPRI SGKPILVLAN KQDKEGALGE ADVIECLSLE
KLVNEHKCLC QIEPCSAISG YGKKIDKSIK KGLYWLLHVI ARDFDALNER
IQKETTEQRA LEEQEKQERA ERVRKLREER KQNEQEQAEL DGTSGLAELD
PEPTNPFQPI ASVIIENEGK LEREKKNQKM EKDSDGCHLK HKMEHEQIET
QGQVNHNGQK NNEFGLVENY KEALTQQLKN EDETDRPSLE SANGKKKTKK
LRMKRNHRVE PLNIDDCAPE SPTPPPPPPP VGWGTPKVTR LPKLEPLGET
HHNDFYRKPL PPLAVPQRPN SDAHDVIS
UCSC uc003drc.4; human. [Q3SXY8-1];

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Human ARL13B ELISA Kit