Human CD83 ELISA Kit

Alternative name
Human hCD83 ELISA Kit;Human B-cell activation protein ELISA Kit;Human cell surface protein HB15 ELISA Kit;Human BL11 ELISA Kit;Human HB15 ELISA Kit;Human CD83 molecule ELISA Kit;Human CD83 antigen ELISA Kit;Human CD83 antigen (activated B lymphocytes, immunoglobulin superfamily) ELISA Kit;Human cell-surface glycoprotein ELISA Kit;
Catalog
E009732
species
Human
GeneCD83
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 83 (CD83). No significant cross-reactivity or interference between Cluster Of Differentiation 83 (CD83) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 1.42pg/mL.
Intended UseHuman CD83 ELISA Kit allows for the in vitro quantitative determination of CD83 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 83 (CD83). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 83 (CD83). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 83 (CD83), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 83 (CD83) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human CD83 antigen Protein information
Uniprot ID CD83_HUMAN
Uniprot AC Q01151; Q5THX9;
UniGene Hs.595133;
GeneID 9308
KEGG hsa:9308;
Human CD83 antigen Protein SEQUENCE
SEQUENCE 205 AA; 23042 MW; 1D77133595F2B852 CRC64;
MSRGLQLLLL SCAYSLAPAT PEVKVACSED VDLPCTAPWD PQVPYTVSWV
KLLEGGEERM ETPQEDHLRG QHYHQKGQNG SFDAPNERPY SLKIRNTTSC
NSGTYRCTLQ DPDGQRNLSG KVILRVTGCP AQRKEETFKK YRAEIVLLLA
LVIFYLTLII FTCKFARLQS IFPDFSKAGM ERAFLPVTSP NKHLGLVTPH
KTELV
UCSC uc003nbi.4; human.;

Human CD83 ELISA Kit

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