Human CD74 ELISA Kit

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  • Alternative name

    Human HLA-DR antigens-associated invariant chain ELISA Kit;Human Ia antigen-associated invariant chain ELISA Kit;Human II ELISA Kit;Human p33 ELISA Kit;Human DHLAG ELISA Kit;Human HLADG ELISA Kit;Human Ia-GAMMA ELISA Kit;Human CD74 molecule ELISA Kit;Human HLA class II histocompatibility antigen gamma chain ELISA Kit;Human CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ELISA Kit;Human CD74 molecule, major histocompatibility complex, class II invariant chain ELISA Kit;Human HLA-DR-gamma ELISA Kit;Human Ia-associated invariant chain ELISA Kit;Human MHC HLA-DR gamma chain ELISA Kit;Human gamma chain of class II antigens ELISA Kit;

  • Catalog
    E009726
  • species
    Human
  • GeneCD74
  • Standard CurveHuman CD74 ELISA Kit
  • Other Species Mouse Cd74 ELISA KitRat Cd74 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD74. No significant cross-reactivity or interference between CD74 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD74 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CD74 ELISA Kit allows for the in vitro quantitative determination of CD74 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: CD74 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD74 antibody and an CD74-HRP conjugate. The assay sample and buffer are incubated together with CD74-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD74 concentration since CD74 from samples and CD74-HRP conjugate compete for the anti-CD74 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD74 from the sample, fewer sites are left to bind CD74-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD74 concentration in each sample is interpolated from this standard curve.
  • Human HLA class II histocompatibility antigen gamma chain Protein information
  • Uniprot ID HG2A_HUMAN
  • Uniprot AC Q8SNA0; Q8WLP6;
  • UniGene Hs.436568;
  • GeneID 972
  • KEGG hsa:972;
  • Human HLA class II histocompatibility antigen gamma chain Protein SEQUENCE
  • SEQUENCE 296 AA; 33516 MW; 27A13F252D5FB91D CRC64;

    MHRRRSRSCR EDQKPVMDDQ RDLISNNEQL PMLGRRPGAP ESKCSRGALY

    TGFSILVTLL LAGQATTAYF LYQQQGRLDK LTVTSQNLQL ENLRMKLPKP

    PKPVSKMRMA TPLLMQALPM GALPQGPMQN ATKYGNMTED HVMHLLQNAD

    PLKVYPPLKG SFPENLRHLK NTMETIDWKV FESWMHHWLL FEMSRHSLEQ

    KPTDAPPKVL TKCQEEVSHI PAVHPGSFRP KCDENGNYLP LQCYGSIGYC

    WCVFPNGTEV PNTRSRGHHN CSESLELEDP SSGLGVTKQD LGPVPM

  • UCSC uc003lsc.4; human. [P04233-1];



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