Human CD4 ELISA Kit

Contact Us
  • Alternative name

    Human T-cell surface antigen T4/Leu-3 ELISA Kit;Human CD4mut ELISA Kit;Human CD4 molecule ELISA Kit;Human T-cell surface glycoprotein CD4 ELISA Kit;Human CD4 antigen (p55) ELISA Kit;Human CD4 receptor ELISA Kit;

  • Catalog
    E009701
  • species
    Human
  • GeneCD4
  • Standard CurveHuman CD4 ELISA Kit
  • Other Species Mouse Cd4 ELISA KitCanine CD4 ELISA KitMonkey CD4 ELISA KitRabbit CD4 ELISA KitRat Cd4 ELISA KitSheep CD4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD4. No significant cross-reactivity or interference between CD4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman CD4 ELISA Kit allows for the in vitro quantitative determination of CD4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: CD4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CD4. Standards or samples are then added to the microtiter plate wells and CD4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CD4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CD4 are added to each well to "sandwich" the CD4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CD4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD4 concentration in each sample is interpolated from this standard curve.
  • Human T-cell surface glycoprotein CD4 Protein information
  • Uniprot ID CD4_HUMAN
  • Uniprot AC P01730; B2R737; D3DUS5; Q4ZGK2; Q5U066; Q9UDE5;
  • UniGene Hs.631659;
  • GeneID 920
  • KEGG hsa:920;
  • Human T-cell surface glycoprotein CD4 Protein SEQUENCE
  • SEQUENCE 458 AA; 51111 MW; 20ED893F9E56D236 CRC64;

    MNRGVPFRHL LLVLQLALLP AATQGKKVVL GKKGDTVELT CTASQKKSIQ

    FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK

    IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS

    PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV

    VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWW QAERASSSKS

    WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLA

    LEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK

    VSKREKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW STPVQPMALI

    VLGGVAGLLL FIGLGIFFCV RCRHRRRQAE RMSQIKRLLS EKKTCQCPHR

    FQKTCSPI

  • UCSC uc001qqv.3; human.;


Related products with Human CD4 ELISA Kit

Inquiry About Human CD4 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!