Human AKAP12 ELISA Kit

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  • Alternative name

    Human AKAP-12 ELISA Kit;Human A-kinase anchor protein 250 kDa ELISA Kit;Human AKAP 250 ELISA Kit;Human Gravin ELISA Kit;Human Myasthenia gravis autoantigen ELISA Kit;Human AKAP250 ELISA Kit;Human SSeCKS ELISA Kit;Human A-kinase anchoring protein 12 ELISA Kit;Human A-kinase anchor protein 12 ELISA Kit;Human A kinase (PRKA) anchor protein 12 ELISA Kit;Human A-kinase anchor protein, 250kDa ELISA Kit;Human Src-Suppressed C Kinase Substrate ELISA Kit;Human kinase scaffold protein gravin ELISA Kit;Human myasthenia gravis autoantigen gravin ELISA Kit;

  • Catalog
    E000097
  • species
    Human
  • GeneAKAP12
  • Other Species Mouse Akap12 ELISA KitRat Akap12 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AKAP12. No significant cross-reactivity or interference between Human AKAP12 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman AKAP12 ELISA Kit allows for the in vitro quantitative determination of AKAP12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Akap12. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Akap12 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Akap12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Akap12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human A-kinase anchor protein 12 Protein information
  • Uniprot ID AKA12_HUMAN
  • Uniprot AC Q02952; O00310; O00498; Q4LE68; Q5SZ80; Q5TGN1; Q68D82; Q99970;
  • UniGene Hs.371240;
  • GeneID 9590
  • KEGG hsa:9590;
  • Human A-kinase anchor protein 12 Protein SEQUENCE
  • SEQUENCE 1782 AA; 191482 MW; DB9DBA4647E6DDD5 CRC64;

    MGAGSSTEQR SPEQPPEGSS TPAEPEPSGG GPSAEAAPDT TADPAIAASD

    PATKLLQKNG QLSTINGVAE QDELSLQEGD LNGQKGALNG QGALNSQEEE

    EVIVTEVGQR DSEDVSKRDS DKEMATKSAV VHDITDDGQE ETPEIIEQIP

    SSESNLEELT QPTESQANDI GFKKVFKFVG FKFTVKKDKT EKPDTVQLLT

    VKKDEGEGAA GAGDHKDPSL GAGEAASKES EPKQSTEKPE ETLKREQSHA

    EISPPAESGQ AVEECKEEGE EKQEKEPSKS AESPTSPVTS ETGSTFKKFF

    TQGWAGWRKK TSFRKPKEDE VEASEKKKEQ EPEKVDTEED GKAEVASEKL

    TASEQAHPQE PAESAHEPRL SAEYEKVELP SEEQVSGSQG PSEEKPAPLA

    TEVFDEKIEV HQEEVVAEVH VSTVEERTEE QKTEVEETAG SVPAEELVEM

    DAEPQEAEPA KELVKLKETC VSGEDPTQGA DLSPDEKVLS KPPEGVVSEV

    EMLSSQERMK VQGSPLKKLF TSTGLKKLSG KKQKGKRGGG DEESGEHTQV

    PADSPDSQEE QKGESSASSP EEPEEITCLE KGLAEVQQDG EAEEGATSDG

    EKKREGVTPW ASFKKMVTPK KRVRRPSESD KEDELDKVKS ATLSSTESTA

    SEMQEEMKGS VEEPKPEEPK RKVDTSVSWE ALICVGSSKK RARRGSSSDE

    EGGPKAMGGD HQKADEAGKD KETGTDGILA GSQEHDPGQG SSSPEQAGSP

    TEGEGVSTWE SFKRLVTPRK KSKSKLEEKS EDSIAGSGVE HSTPDTEPGK

    EESWVSIKKF IPGRRKKRPD GKQEQAPVED AGPTGANEDD SDVPAVVPLS

    EYDAVEREKM EAQQAQKSAE QPEQKAATEV SKELSESQVH MMAAAVADGT

    RAATIIEERS PSWISASVTE PLEQVEAEAA LLTEEVLERE VIAEEEPPTV

    TEPLPENREA RGDTVVSEAE LTPEAVTAAE TAGPLGAEEG TEASAAEETT

    EMVSAVSQLT DSPDTTEEAT PVQEVEGGVP DIEEQERRTQ EVLQAVAEKV

    KEESQLPGTG GPEDVLQPVQ RAEAERPEEQ AEASGLKKET DVVLKVDAQE

    AKTEPFTQGK VVGQTTPESF EKAPQVTESI ESSELVTTCQ AETLAGVKSQ

    EMVMEQAIPP DSVETPTDSE TDGSTPVADF DAPGTTQKDE IVEIHEENEV

    ASGTQSGGTE AEAVPAQKER PPAPSSFVFQ EETKEQSKME DTLEHTDKEV

    SVETVSILSK TEGTQEADQY ADEKTKDVPF FEGLEGSIDT GITVSREKVT

    EVALKGEGTE EAECKKDDAL ELQSHAKSPP SPVEREMVVQ VEREKTEAEP

    THVNEEKLEH ETAVTVSEEV SKQLLQTVNV PIIDGAKEVS SLEGSPPPCL

    GQEEAVCTKI QVQSSEASFT LTAAAEEEKV LGETANILET GETLEPAGAH

    LVLEEKSSEK NEDFAAHPGE DAVPTGPDCQ AKSTPVIVSA TTKKGLSSDL

    EGEKTTSLKW KSDEVDEQVA CQEVKVSVAI EDLEPENGIL ELETKSSKLV

    QNIIQTAVDQ FVRTEETATE MLTSELQTQA HVIKADSQDA GQETEKEGEE

    PQASAQDETP ITSAKEESES TAVGQAHSDI SKDMSEASEK TMTVEVEGST

    VNDQQLEEVV LPSEEEGGGA GTKSVPEDDG HALLAERIEK SLVEPKEDEK

    GDDVDDPENQ NSALADTDAS GGLTKESPDT NGPKQKEKED AQEVELQEGK

    VHSESDKAIT PQAQEELQKQ ERESAKSELT ES

  • UCSC uc003qoe.5; human. [Q02952-1];


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