SpecificityThis assay has high sensitivity and excellent specificity for detection of CD24. No significant cross-reactivity or interference between CD24 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD24 and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman CD24 ELISA Kit allows for the in vitro quantitative determination of CD24 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalIntended Uses||This CD24 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD24. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: CD24 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD24 antibody and an CD24-HRP conjugate. The assay sample and buffer are incubated together with CD24-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD24 concentration since CD24 from samples and CD24-HRP conjugate compete for the anti-CD24 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD24 from the sample, fewer sites are left to bind CD24-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD24 concentration in each sample is interpolated from this standard curve.