Human CD24 ELISA Kit

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  • Alternative name

    Human Small cell lung carcinoma cluster 4 antigen ELISA Kit;Human CD24A ELISA Kit;Human CD24 molecule ELISA Kit;Human signal transducer CD24 ELISA Kit;Human CD24 antigen (small cell lung carcinoma cluster 4 antigen) ELISA Kit;

  • Catalog
    E009683
  • species
    Human
  • GeneCD24
  • Standard CurveHuman CD24 ELISA Kit
  • Other Species Mouse Cd24 ELISA KitRat Cd24 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CD24. No significant cross-reactivity or interference between CD24 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD24 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman CD24 ELISA Kit allows for the in vitro quantitative determination of CD24 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Intended Uses||This CD24 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CD24. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CD24 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD24 antibody and an CD24-HRP conjugate. The assay sample and buffer are incubated together with CD24-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD24 concentration since CD24 from samples and CD24-HRP conjugate compete for the anti-CD24 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD24 from the sample, fewer sites are left to bind CD24-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD24 concentration in each sample is interpolated from this standard curve.
  • Human Signal transducer CD24 Protein information
  • Uniprot ID CD24_HUMAN
  • Uniprot AC P25063; Q16257; Q53XS0;
  • UniGene Hs.644105; Hs.712050;
  • GeneID 100133941
  • KEGG hsa:100133941;
  • Human Signal transducer CD24 Protein SEQUENCE
  • SEQUENCE 80 AA; 8097 MW; DB1988B684DEC62F CRC64;

    MGRAMVARLG LGLLLLALLL PTQIYSSETT TGTSSNSSQS TSNTGLAPNP

    TNATTKAAGG ALQSTASLFV VSLSLLHLYS



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