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Human CLASP2 ELISA Kit

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  • Alternative name

    Human Cytoplasmic linker-associated protein 2 ELISA Kit;Human protein Orbit homolog 2 ELISA Kit;Human hOrbit2 ELISA Kit;Human KIAA0627 ELISA Kit;Human cytoplasmic linker associated protein 2 ELISA Kit;Human CLIP-associating protein 2 ELISA Kit;Human CLIP-associating protein CLASP2 ELISA Kit;Human multiple asters (Mast)-like homolog 2 ELISA Kit;
  • Catalog
    E009653
  • species
    Human
  • GeneCLASP2
  • Other Species Mouse Clasp2 ELISA KitRat Clasp2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CLASP2. No significant cross-reactivity or interference between CLASP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLASP2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CLASP2 ELISA Kit allows for the in vitro quantitative determination of CLASP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the Assay: CLASP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLASP2 antibody and an CLASP2-HRP conjugate. The assay sample and buffer are incubated together with CLASP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLASP2 concentration since CLASP2 from samples and CLASP2-HRP conjugate compete for the anti-CLASP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLASP2 from the sample, fewer sites are left to bind CLASP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLASP2 concentration in each sample is interpolated from this standard curve.
  • Human CLIP-associating protein 2 Protein information
  • Uniprot ID CLAP2_HUMAN
  • Uniprot AC Q9NSZ2;
  • UniGene Hs.108614;
  • GeneID 23122
  • KEGG hsa:23122;
  • Human CLIP-associating protein 2 Protein SEQUENCE

  • SEQUENCE 1294 AA; 141133 MW; E4FF7E06049E65E5 CRC64;

    MAMGDDKSFD DEESVDGNRP SSAASAFKVP APKTSGNPAN SARKPGSAGG

    PKVGGASKEG GAGAVDEDDF IKAFTDVPSI QIYSSRELEE TLNKIREILS

    DDKHDWDQRA NALKKIRSLL VAGAAQYDCF FQHLRLLDGA LKLSAKDLRS

    QVVREACITV AHLSTVLGNK FDHGAEAIVP TLFNLVPNSA KVMATSGCAA

    IRFIIRHTHV PRLIPLITSN CTSKSVPVRR RSFEFLDLLL QEWQTHSLER

    HAAVLVETIK KGIHDADAEA RVEARKTYMG LRNHFPGEAE TLYNSLEPSY

    QKSLQTYLKS SGSVASLPQS DRSSSSSQES LNRPFSSKWS TANPSTVAGR

    VSAGSSKASS LPGSLQRSRS DIDVNAAAGA KAHHAAGQSV RRGRLGAGAL

    NAGSYASLED TSDKLDGTAS EDGRVRAKLS APLAGMGNAK ADSRGRSRTK

    MVSQSQPGSR SGSPGRVLTT TALSTVSSGV QRVLVNSASA QKRSKIPRSQ

    GCSREASPSR LSVARSSRIP RPSVSQGCSR EASRESSRDT SPVRSFQPLA

    SRHHSRSTGA LYAPEVYGAS GPGYGISQSS RLSSSVSAMR VLNTGSDVEE

    AVADALKKPA RRRYESYGMH SDDDANSDAS SACSERSYSS RNGSIPTYMR

    QTEDVAEVLN RCASSNWSER KEGLLGLQNL LKNQRTLSRV ELKRLCEIFT

    RMFADPHGKR VFSMFLETLV DFIQVHKDDL QDWLFVLLTQ LLKKMGADLL

    GSVQAKVQKA LDVTRESFPN DLQFNILMRF TVDQTQTPSL KVKVAILKYI

    ETLAKQMDPG DFINSSETRL AVSRVITWTT EPKSSDVRKA AQSVLISLFE

    LNTPEFTMLL GALPKTFQDG ATKLLHNHLR NTGNGTQSSM GSPLTRPTPR

    SPANWSSPLT SPTNTSQNTL SPSAFDYDTE NMNSEDIYSS LRGVTEAIQN

    FSFRSQEDMN EPLKRDSKKD DGDSMCGGPG MSDPRAGGDA TDSSQTALDN

    KASLLHSMPT HSSPRSRDYN PYNYSDSISP FNKSALKEAM FDDDADQFPD

    DLSLDHSDLV AELLKELSNH NERVEERKIA LYELMKLTQE ESFSVWDEHF

    KTILLLLLET LGDKEPTIRA LALKVLREIL RHQPARFKNY AELTVMKTLE

    AHKDPHKEVV RSAEEAASVL ATSISPEQCI KVLCPIIQTA DYPINLAAIK

    MQTKVIERVS KETLNLLLPE IMPGLIQGYD NSESSVRKAC VFCLVAVHAV

    IGDELKPHLS QLTGSKMKLL NLYIKRAQTG SGGADPTTDV SGQS

  • UCSC uc003cfv.4; human. [O75122-1];


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