Human CPSF1 ELISA Kit

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  • Alternative name

    Human cleavage and polyadenylation specificity factor 160 kDa subunit ELISA Kit;Human CPSF 160 kDa subunit ELISA Kit;Human CPSF160 ELISA Kit;Human HSU37012 ELISA Kit;Human P/cl.18 ELISA Kit;Human cleavage and polyadenylation specific factor 1 ELISA Kit;Human cleavage and polyadenylation specificity factor subunit 1 ELISA Kit;Human cleavage and polyadenylation specific factor 1, 160kDa ELISA Kit;Human polyadenylation specificity factor ELISA Kit;

  • Catalog
    E009635
  • species
    Human
  • GeneCPSF1
  • Standard CurveHuman CPSF1 ELISA Kit
  • Other Species Mouse Cpsf1 ELISA KitBovine CPSF1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cleavage And Polyadenylation Specific Factor 1 (CPSF1). No significant cross-reactivity or interference between Cleavage And Polyadenylation Specific Factor 1 (CPSF1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.129ng/mL.
  • Intended UseHuman CPSF1 ELISA Kit allows for the in vitro quantitative determination of CPSF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cleavage And Polyadenylation Specific Factor 1 (CPSF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cleavage And Polyadenylation Specific Factor 1 (CPSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cleavage And Polyadenylation Specific Factor 1 (CPSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cleavage and polyadenylation specificity factor subunit 1 Protein information
  • Uniprot ID CPSF1_HUMAN
  • Uniprot AC Q10570; Q96AF0;
  • UniGene Hs.493202;
  • GeneID 29894
  • KEGG hsa:29894;
  • Human Cleavage and polyadenylation specificity factor subunit 1 Protein SEQUENCE
  • SEQUENCE 1443 AA; 160884 MW; 7E1DF4D8A93487A4 CRC64;

    MYAVYKQAHP PTGLEFSMYC NFFNNSERNL VVAGTSQLYV YRLNRDAEAL

    TKNDRSTEGK AHREKLELAA SFSFFGNVMS MASVQLAGAK RDALLLSFKD

    AKLSVVEYDP GTHDLKTLSL HYFEEPELRD GFVQNVHTPR VRVDPDGRCA

    AMLVYGTRLV VLPFRRESLA EEHEGLVGEG QRSSFLPSYI IDVRALDEKL

    LNIIDLQFLH GYYEPTLLIL FEPNQTWPGR VAVRQDTCSI VAISLNITQK

    VHPVIWSLTS LPFDCTQALA VPKPIGGVVV FAVNSLLYLN QSVPPYGVAL

    NSLTTGTTAF PLRTQEGVRI TLDCAQATFI SYDKMVISLK GGEIYVLTLI

    TDGMRSVRAF HFDKAAASVL TTSMVTMEPG YLFLGSRLGN SLLLKYTEKL

    QEPPASAVRE AADKEEPPSK KKRVDATAGW SAAGKSVPQD EVDEIEVYGS

    EAQSGTQLAT YSFEVCDSIL NIGPCANAAV GEPAFLSEEF QNSPEPDLEI

    VVCSGHGKNG ALSVLQKSIR PQVVTTFELP GCYDMWTVIA PVRKEEEDNP

    KGEGTEQEPS TTPEADDDGR RHGFLILSRE DSTMILQTGQ EIMELDTSGF

    ATQGPTVFAG NIGDNRYIVQ VSPLGIRLLE GVNQLHFIPV DLGAPIVQCA

    VADPYVVIMS AEGHVTMFLL KSDSYGGRHH RLALHKPPLH HQSKVITLCL

    YRDLSGMFTT ESRLGGARDE LGGRSGPEAE GLGSETSPTV DDEEEMLYGD

    SGSLFSPSKE EARRSSQPPA DRDPAPFRAE PTHWCLLVRE NGTMEIYQLP

    DWRLVFLVKN FPVGQRVLVD SSFGQPTTQG EARREEATRQ GELPLVKEVL

    LVALGSRQSR PYLLVHVDQE LLIYEAFPHD SQLGQGNLKV RFKKVPHNIN

    FREKKPKPSK KKAEGGGAEE GAGARGRVAR FRYFEDIYGY SGVFICGPSP

    HWLLVTGRGA LRLHPMAIDG PVDSFAPFHN VNCPRGFLYF NRQGELRISV

    LPAYLSYDAP WPVRKIPLRC TAHYVAYHVE SKVYAVATST NTPCARIPRM

    TGEEKEFETI ERDERYIHPQ QEAFSIQLIS PVSWEAIPNA RIELQEWEHV

    TCMKTVSLRS EETVSGLKGY VAAGTCLMQG EEVTCRGRIL IMDVIEVVPE

    PGQPLTKNKF KVLYEKEQKG PVTALCHCNG HLVSAIGQKI FLWSLRASEL

    TGMAFIDTQL YIHQMISVKN FILAADVMKS ISLLRYQEES KTLSLVSRDA

    KPLEVYSVDF MVDNAQLGFL VSDRDRNLMV YMYLPEAKES FGGMRLLRRA

    DFHVGAHVNT FWRTPCRGAT EGLSKKSVVW ENKHITWFAT LDGGIGLLLP

    MQEKTYRRLL MLQNALTTML PHHAGLNPRA FRMLHVDRRT LQNAVRNVLD

    GELLNRYLYL STMERSELAK KIGTTPDIIL DDLLETDRVT AHF

  • UCSC uc003zcj.3; human.;


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