Human CLDN15 ELISA Kit

Alternative name
Human claudin 15 ELISA Kit;Human claudin-15 ELISA Kit;
Catalog
E009575
species
Human
GeneCLDN15
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLDN15. No significant cross-reactivity or interference between CLDN15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN15 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CLDN15 ELISA Kit allows for the in vitro quantitative determination of CLDN15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: CLDN15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLDN15 antibody and an CLDN15-HRP conjugate. The assay sample and buffer are incubated together with CLDN15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLDN15 concentration since CLDN15 from samples and CLDN15-HRP conjugate compete for the anti-CLDN15 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLDN15 from the sample, fewer sites are left to bind CLDN15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN15 concentration in each sample is interpolated from this standard curve.
Human Claudin-15 Protein information
Uniprot ID CLD15_HUMAN
Uniprot AC P56746; B3KPB5;
UniGene Hs.38738;
GeneID 24146
KEGG hsa:24146;
Human Claudin-15 Protein SEQUENCE
SEQUENCE 228 AA; 24356 MW; D502E2CD9116F4B1 CRC64;
MSMAVETFGF FMATVGLLML GVTLPNSYWR VSTVHGNVIT TNTIFENLWF
SCATDSLGVY NCWEFPSMLA LSGYIQACRA LMITAILLGF LGLLLGIAGL
RCTNIGGLEL SRKAKLAATA GALHILAGIC GMVAISWYAF NITRDFFDPL
YPGTKYELGP ALYLGWSASL ISILGGLCLC SACCCGSDED PAASARRPYQ
APVSVMPVAT SDQEGDSSFG KYGRNAYV
UCSC uc003uyg.2; human.;

Category

Human CLDN15 ELISA Kit

Human claudin 15 ELISA Kit;Human claudin-15 ELISA Kit;