Human SNAP91 ELISA Kit

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  • Alternative name

    Human 91 kDa synaptosomal-associated protein ELISA Kit;Human clathrin coat-associated protein AP180 ELISA Kit;Human phosphoprotein F1-20 ELISA Kit;Human KIAA0656 ELISA Kit;Human AP180 ELISA Kit;Human CALM ELISA Kit;Human synaptosome associated protein 91 ELISA Kit;Human clathrin coat assembly protein AP180 ELISA Kit;Human assembly protein, 180kDa ELISA Kit;Human synaptosomal-associated protein, 91kDa homolog ELISA Kit;Human synaptosome associated protein 91kDa ELISA Kit;

  • Catalog
    E009551
  • species
    Human
  • GeneSNAP91
  • Standard CurveHuman SNAP91 ELISA Kit
  • Other Species Mouse Snap91 ELISA KitRat Snap91 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SNAP91. No significant cross-reactivity or interference between SNAP91 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SNAP91 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman SNAP91 ELISA Kit allows for the in vitro quantitative determination of SNAP91 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This SNAP91 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SNAP91. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||SNAP91 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SNAP91 antibody and an SNAP91-HRP conjugate. The assay sample and buffer are incubated together with SNAP91-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SNAP91 concentration since SNAP91 from samples and SNAP91-HRP conjugate compete for the anti-SNAP91 antibody binding site. Since the number of sites is limited, as more sites are occupied by SNAP91 from the sample, fewer sites are left to bind SNAP91-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SNAP91 concentration in each sample is interpolated from this standard curve.
  • Human Clathrin coat assembly protein AP180 Protein information
  • Uniprot ID AP180_HUMAN
  • Uniprot AC O60641; A8K0L7; E5RI02; Q5JX13; Q68DL9; Q6P9D3; Q9NTY7;
  • UniGene Hs.368046;
  • GeneID 9892
  • KEGG hsa:9892;
  • Human Clathrin coat assembly protein AP180 Protein SEQUENCE
  • SEQUENCE 907 AA; 92502 MW; 23959C2B54F5EBF1 CRC64;

    MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN

    ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR

    NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK

    GADGVMRTMA PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL

    FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF

    LKVAEQVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS

    PLSKSSPATT VTSPNSTPAK TIDTSPPVDL FATASAAVPV STSKPSSDLL

    DLQPDFSSGG AAAAAAPAPP PPAGGATAWG DLLGEDSLAA LSSVPSEAQI

    SDPFAPEPTP PTTTAEIATA SASASTTTTV TAVTAEVDLF GDAFAASPGE

    APAASEGAAA PATPTPVAAA LDACSGNDPF APSEGSAEAA PELDLFAMKP

    PETSVPVVTP TASTAPPVPA TAPSPAPAVA AAAAATTAAT AAATTTTTTS

    AATATTAPPA LDIFGDLFES TPEVAAAPKP DAAPSIDLFS TDAFSSPPQG

    ASPVPESSLT ADLLSVDAFA APSPATTASP AKVDSSGVID LFGDAFGSSA

    SEPQPASQAA SSSSASADLL AGFGGSFMAP SPSPVTPAQN NLLQPNFEAA

    FGTTPSTSSS SSFDPSVFDG LGDLLMPTMA PAGQPAPVSM VPPSPAMAAS

    KALGSDLDSS LASLVGNLGI SGTTTKKGDL QWNAGEKKLT GGANWQPKVA

    PATWSAGVPP SAPLQGAVPP TSSVPPVAGA PSVGQPGAGF GMPPAGTGMP

    MMPQQPVMFA QPMMRPPFGA AAVPGTQLSP SPTPASQSPK KPPAKDPLAD

    LNIKDFL

  • UCSC uc003pkc.4; human. [O60641-1];



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