Human CROP ELISA Kit

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  • Alternative name

    Luc7-like protein 3 ELISA KIT; Cisplatin resistance-associated-overexpressed protein ELISA KIT; Luc7A ELISA KIT; Okadaic acid-inducible phosphoprotein OA48-18 ELISA KIT; cAMP regulatory element-associated protein 1 ELISA KIT; CRE-associated protein 1 ELISA KIT; CREAP-1LUC7L3 ELISA KIT; CREAP1 ELISA KIT; CROP ELISA KIT; O48 ELISA KIT; CRE-associated protein 1 ELISA KIT; CREAP-1 ELISA KIT

  • Catalog
    E009483
  • species
    Human
  • GeneCROP
  • Standard CurveHuman CROP ELISA Kit
  • Other Species Mouse CROP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CROP ELISA Kit allows for the in vitro quantitative determination of CROP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: CROP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CROP antibody and an CROP-HRP conjugate. The assay sample and buffer are incubated together with CROP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CROP concentration since CROP from samples and CROP-HRP conjugate compete for the anti-CROP antibody binding site. Since the number of sites is limited, as more sites are occupied by CROP from the sample, fewer sites are left to bind CROP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CROP concentration in each sample is interpolated from this standard curve.


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