Human CMA1 ELISA Kit

Alternative name
Human alpha-chymase ELISA Kit;Human mast cell protease I ELISA Kit;Human CYH ELISA Kit;Human CYM ELISA Kit;Human MCT1 ELISA Kit;Human chymase ELISA Kit;Human chymase 1 ELISA Kit;Human chymase 1 preproprotein transcript E ELISA Kit;Human chymase 1 preproprotein transcript I ELISA Kit;Human chymase 1, mast cell ELISA Kit;Human chymase, heart ELISA Kit;Human chymase, mast cell ELISA Kit;
Catalog
E009439
species
Human
GeneCMA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human mast cell chymase. No significant cross-reactivity or interference between human mast cell chymase and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human mast cell chymase is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was dete
Intended UseHuman CMA1 ELISA Kit allows for the in vitro quantitative determination of CMA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mast cell chymase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mast cell chymase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mast cell chymase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mast cell chymase bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Chymase Protein information
Uniprot ID CMA1_HUMAN
Uniprot AC P23946; B5BUM8; Q16018; Q3SY36; Q3SY37; Q9UDH5;
UniGene Hs.135626;
GeneID 1215
KEGG hsa:1215;
Human Chymase Protein SEQUENCE
SEQUENCE 247 AA; 27325 MW; DC1464A049ED6B00 CRC64;
MLLLPLPLLL FLLCSRAEAG EIIGGTECKP HSRPYMAYLE IVTSNGPSKF
CGGFLIRRNF VLTAAHCAGR SITVTLGAHN ITEEEDTWQK LEVIKQFRHP
KYNTSTLHHD IMLLKLKEKA SLTLAVGTLP FPSQFNFVPP GRMCRVAGWG
RTGVLKPGSD TLQEVKLRLM DPQACSHFRD FDHNLQLCVG NPRKTKSAFK
GDSGGPLLCA GVAQGIVSYG RSDAKPPAVF TRISHYRPWI NQILQAN
UCSC uc001wpp.2; human. [P23946-1];

Human CMA1 ELISA Kit

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