Human ADFP ELISA Kit

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  • Alternative name

    Perilipin-2 ELISA KIT; Adipophilin ELISA KIT; Adipose differentiation-related protein ELISA KIT; ADRPPLIN2 ELISA KIT; ADFP ELISA KIT; ADRP ELISA KIT

  • Catalog
    E000939
  • species
    Human
  • GeneADFP
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ADFP. No significant cross-reactivity or interference between Human ADFP and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.78 ng/ml
  • Intended UseHuman ADFP ELISA Kit allows for the in vitro quantitative determination of ADFP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Introduction: Adipose differentiation-related protein, is a protein which in humans is encoded by the ADRP gene. Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADRP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ADRP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADRP in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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