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Alternative name
Adiponectin ELISA KIT; 30 kDa adipocyte complement-related protein ELISA KIT; Adipocyte complement-related 30 kDa protein ELISA KIT; ACRP30 ELISA KIT; Adipocyte, C1q and collagen domain-containing protein ELISA KIT; Adipose most abundant gene transcript 1 protein ELISA KIT; apM-1 ELISA KIT; Gelatin-binding proteinADIPOQ ELISA KIT; ACDC ELISA KIT; ACRP30 ELISA KIT; APM1 ELISA KIT; GBP28 ELISA KIT; ACRP30 ELISA KIT; apM-1 ELISA KIT
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Catalog
E000920
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species
Human
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GeneADP
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Standard Curve
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Other Species
Human ADP/Acrp30 ELISA KitHuman ADP-HMW ELISA KitHuman HMW-ADP ELISA KitMouse ADP/Acrp30 ELISA KitMouse ADP ELISA KitChicken NAD(P)(+)--arginine ADP-ribosyltransferase 1 ELISA KitChicken NAD(P)(+)--arginine ADP-ribosyltransferase 2 ELISA KitHuman Mono [ADP-ribose] polymerase PARP16 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Adiponectin (ADP).
No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.062ng/mL.
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Intended UseHuman ADP ELISA Kit allows for the in vitro quantitative determination of ADP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin (ADP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adiponectin (ADP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adiponectin (ADP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adiponectin (ADP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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