Human ADIG ELISA Kit

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  • Alternative name

    Human SMAF1 ELISA Kit;Human adipogenin ELISA Kit;Human adipogenesis associated ELISA Kit;Human small adipocyte factor 1 (SMAF1) ELISA Kit;

  • Catalog
    E000911
  • species
    Human
  • GeneADIG
  • Standard CurveHuman ADIG ELISA Kit
  • Other Species Mouse Adig ELISA KitBovine ADIG ELISA KitPorcine ADIG ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of adipogenin. No significant cross-reactivity or interference between adipogenin and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of adipogenin is typically less than 0.56ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseHuman ADIG ELISA Kit allows for the in vitro quantitative determination of ADIG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to adipogenin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to adipogenin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain adipogenin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of adipogenin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Adipogenin Protein information
  • Uniprot ID ADIG_HUMAN
  • Uniprot AC Q0VDE8;
  • UniGene Hs.368028;
  • GeneID 149685
  • KEGG hsa:149685;
  • Human Adipogenin Protein SEQUENCE
  • SEQUENCE 80 AA; 9465 MW; 7DC102D170BBF86F CRC64;

    MKYPLMPLVN DLTFSFLVFW FCLPVGLLLL LIIWLRFLLS QDSEENDSSV

    CLDWEPWSKG PAEFCWKGTL HGQEKERPCW

  • UCSC uc002xjb.1; human.;




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