Human APMAP ELISA Kit

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  • Alternative name

    Human protein BSCv ELISA Kit;Human C20orf3 ELISA Kit;Human BSCv ELISA Kit;Human adipocyte plasma membrane associated protein ELISA Kit;Human adipocyte plasma membrane-associated protein ELISA Kit;

  • Catalog
    E000905
  • species
    Human
  • GeneAPMAP
  • Standard CurveHuman APMAP ELISA Kit
  • Other Species Mouse Apmap ELISA KitBovine APMAP ELISA KitChicken APMAP ELISA KitRat Apmap ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Adipocyte Plasma Membrane Associated Protein (APMAP). No significant cross-reactivity or interference between Adipocyte Plasma Membrane Associated Protein (APMAP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman APMAP ELISA Kit allows for the in vitro quantitative determination of APMAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adipocyte Plasma Membrane Associated Protein (APMAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adipocyte Plasma Membrane Associated Protein (APMAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adipocyte Plasma Membrane Associated Protein (APMAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adipocyte Plasma Membrane Associated Protein (APMAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Adipocyte plasma membrane-associated protein Protein information
  • Uniprot ID APMAP_HUMAN
  • Uniprot AC Q9HDC9; A8K514; B4DXG1; Q6UVZ8; Q9GZS8; Q9NUB2;
  • UniGene Hs.472330;
  • GeneID 57136
  • KEGG hsa:57136;
  • Human Adipocyte plasma membrane-associated protein Protein SEQUENCE
  • SEQUENCE 416 AA; 46480 MW; C88E2DF2DF6093C4 CRC64;

    MSEADGLRQR RPLRPQVVTD DDGQAPEAKD GSSFSGRVFR VTFLMLAVSL

    TVPLLGAMML LESPIDPQPL SFKEPPLLLG VLHPNTKLRQ AERLFENQLV

    GPESIAHIGD VMFTGTADGR VVKLENGEIE TIARFGSGPC KTRDDEPVCG

    RPLGIRAGPN GTLFVADAYK GLFEVNPWKR EVKLLLSSET PIEGKNMSFV

    NDLTVTQDGR KIYFTDSSSK WQRRDYLLLV MEGTDDGRLL EYDTVTREVK

    VLLDQLRFPN GVQLSPAEDF VLVAETTMAR IRRVYVSGLM KGGADLFVEN

    MPGFPDNIRP SSSGGYWVGM STIRPNPGFS MLDFLSERPW IKRMIFKLFS

    QETVMKFVPR YSLVLELSDS GAFRRSLHDP DGLVATYISE VHEHDGHLYL

    GSFRSPFLCR LSLQAV

  • UCSC uc002wty.4; human. [Q9HDC9-1];




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