Human ADAMTS4 ELISA Kit

Alternative name
Human ADAM-TS 4 ELISA Kit;Human ADAM-TS4 ELISA Kit;Human ADAMTS-4 ELISA Kit;Human ADMP-1 ELISA Kit;Human aggrecanase-1 ELISA Kit;Human KIAA0688 ELISA Kit;Human ADAMTS-2 ELISA Kit;Human ADAM metallopeptidase with thrombospondin type 1 motif 4 ELISA Kit;Human A disintegrin and metalloproteinase with thrombospondin motifs 4 ELISA Kit;Human a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 ELISA Kit;
Catalog
E000088
species
Human
GeneADAMTS4
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADAMT1M4. No significant cross-reactivity or interference between ADAMT1M4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMT1M4 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman ADAMTS4 ELISA Kit allows for the in vitro quantitative determination of ADAMTS4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: ADAMT1M4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMT1M4 antibody and an ADAMT1M4-HRP conjugate. The assay sample and buffer are incubated together with ADAMT1M4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMT1M4 concentration since ADAMT1M4 from samples and ADAMT1M4-HRP conjugate compete for the anti-ADAMT1M4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMT1M4 from the sample, fewer sites are left to bind ADAMT1M4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMT1M4 concentration in each sample is interpolated from this standard curve.
Human A disintegrin and metalloproteinase with thrombospondin motifs 4 Protein information
Uniprot ID ATS4_HUMAN
Uniprot AC O75173; Q2HYD0; Q5VTW2; Q6P4Q8; Q6UWA8; Q9UN83;
UniGene Hs.211604;
GeneID 9507
KEGG hsa:9507;
Human A disintegrin and metalloproteinase with thrombospondin motifs 4 Protein SEQUENCE
SEQUENCE 837 AA; 90197 MW; 0C05299D7FB23A8D CRC64;
MSQTGSHPGR GLAGRWLWGA QPCLLLPIVP LSWLVWLLLL LLASLLPSAR
LASPLPREEE IVFPEKLNGS VLPGSGAPAR LLCRLQAFGE TLLLELEQDS
GVQVEGLTVQ YLGQAPELLG GAEPGTYLTG TINGDPESVA SLHWDGGALL
GVLQYRGAEL HLQPLEGGTP NSAGGPGAHI LRRKSPASGQ GPMCNVKAPL
GSPSPRPRRA KRFASLSRFV ETLVVADDKM AAFHGAGLKR YLLTVMAAAA
KAFKHPSIRN PVSLVVTRLV ILGSGEEGPQ VGPSAAQTLR SFCAWQRGLN
TPEDSDPDHF DTAILFTRQD LCGVSTCDTL GMADVGTVCD PARSCAIVED
DGLQSAFTAA HELGHVFNML HDNSKPCISL NGPLSTSRHV MAPVMAHVDP
EEPWSPCSAR FITDFLDNGY GHCLLDKPEA PLHLPVTFPG KDYDADRQCQ
LTFGPDSRHC PQLPPPCAAL WCSGHLNGHA MCQTKHSPWA DGTPCGPAQA
CMGGRCLHMD QLQDFNIPQA GGWGPWGPWG DCSRTCGGGV QFSSRDCTRP
VPRNGGKYCE GRRTRFRSCN TEDCPTGSAL TFREEQCAAY NHRTDLFKSF
PGPMDWVPRY TGVAPQDQCK LTCQAQALGY YYVLEPRVVD GTPCSPDSSS
VCVQGRCIHA GCDRIIGSKK KFDKCMVCGG DGSGCSKQSG SFRKFRYGYN
NVVTIPAGAT HILVRQQGNP GHRSIYLALK LPDGSYALNG EYTLMPSPTD
VVLPGAVSLR YSGATAASET LSGHGPLAQP LTLQVLVAGN PQDTRLRYSF
FVPRPTPSTP RPTPQDWLHR RAQILEILRR RPWAGRK
UCSC uc001fyt.5; human. [O75173-1];

Category

Human ADAMTS4 ELISA Kit