Human ADSL ELISA Kit

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  • Alternative name

    Human ADSL ELISA Kit;Human ASL ELISA Kit;Human adenylosuccinase ELISA Kit;Human ASASE ELISA Kit;Human AMPS ELISA Kit;Human adenylosuccinate lyase ELISA Kit;

  • Catalog
    E000877
  • species
    Human
  • GeneADSL
  • Standard CurveHuman ADSL ELISA Kit
  • Other Species Mouse Adsl ELISA KitBovine ADSL ELISA KitChicken ADSL ELISA KitMonkey ADSL ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ADSL. No significant cross-reactivity or interference between ADSL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADSL and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ADSL ELISA Kit allows for the in vitro quantitative determination of ADSL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Priciple of the assay: ADSL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADSL antibody and an ADSL-HRP conjugate. The assay sample and buffer are incubated together with ADSL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADSL concentration since ADSL from samples and ADSL-HRP conjugate compete for the anti-ADSL antibody binding site. Since the number of sites is limited, as more sites are occupied by ADSL from the sample, fewer sites are left to bind ADSL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADSL concentration in each sample is interpolated from this standard curve.
  • Human Adenylosuccinate lyase Protein information
  • Uniprot ID PUR8_HUMAN
  • Uniprot AC P30566; B0QY76; O75495; Q5TI34;
  • UniGene Hs.75527;
  • GeneID 158
  • KEGG hsa:158;
  • Human Adenylosuccinate lyase Protein SEQUENCE
  • SEQUENCE 484 AA; 54889 MW; 7AA3A0A2C681FD94 CRC64;

    MAAGGDHGSP DSYRSPLASR YASPEMCFVF SDRYKFRTWR QLWLWLAEAE

    QTLGLPITDE QIQEMKSNLE NIDFKMAAEE EKRLRHDVMA HVHTFGHCCP

    KAAGIIHLGA TSCYVGDNTD LIILRNALDL LLPKLARVIS RLADFAKERA

    SLPTLGFTHF QPAQLTTVGK RCCLWIQDLC MDLQNLKRVR DDLRFRGVKG

    TTGTQASFLQ LFEGDDHKVE QLDKMVTEKA GFKRAFIITG QTYTRKVDIE

    VLSVLASLGA SVHKICTDIR LLANLKEMEE PFEKQQIGSS AMPYKRNPMR

    SERCCSLARH LMTLVMDPLQ TASVQWFERT LDDSANRRIC LAEAFLTADT

    ILNTLQNISE GLVVYPKVIE RRIRQELPFM ATENIIMAMV KAGGSRQDCH

    EKIRVLSQQA ASVVKQEGGD NDLIERIQVD AYFSPIHSQL DHLLDPSSFT

    GRASQQVQRF LEEEVYPLLK PYESVMKVKA ELCL

  • UCSC uc003ays.5; human. [P30566-1];


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