Human ADSL ELISA Kit

Alternative name
Human ADSL ELISA Kit;Human ASL ELISA Kit;Human adenylosuccinase ELISA Kit;Human ASASE ELISA Kit;Human AMPS ELISA Kit;Human adenylosuccinate lyase ELISA Kit;
Catalog
E000877
species
Human
GeneADSL
Standard Curve
Other Species Mouse Adsl ELISA Kit Bovine ADSL ELISA Kit Chicken ADSL ELISA Kit Monkey ADSL ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADSL. No significant cross-reactivity or interference between ADSL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADSL and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ADSL ELISA Kit allows for the in vitro quantitative determination of ADSL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: ADSL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADSL antibody and an ADSL-HRP conjugate. The assay sample and buffer are incubated together with ADSL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADSL concentration since ADSL from samples and ADSL-HRP conjugate compete for the anti-ADSL antibody binding site. Since the number of sites is limited, as more sites are occupied by ADSL from the sample, fewer sites are left to bind ADSL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADSL concentration in each sample is interpolated from this standard curve.
Human Adenylosuccinate lyase Protein information
Uniprot ID PUR8_HUMAN
Uniprot AC P30566; B0QY76; O75495; Q5TI34;
UniGene Hs.75527;
GeneID 158
KEGG hsa:158;
Human Adenylosuccinate lyase Protein SEQUENCE
SEQUENCE 484 AA; 54889 MW; 7AA3A0A2C681FD94 CRC64;
MAAGGDHGSP DSYRSPLASR YASPEMCFVF SDRYKFRTWR QLWLWLAEAE
QTLGLPITDE QIQEMKSNLE NIDFKMAAEE EKRLRHDVMA HVHTFGHCCP
KAAGIIHLGA TSCYVGDNTD LIILRNALDL LLPKLARVIS RLADFAKERA
SLPTLGFTHF QPAQLTTVGK RCCLWIQDLC MDLQNLKRVR DDLRFRGVKG
TTGTQASFLQ LFEGDDHKVE QLDKMVTEKA GFKRAFIITG QTYTRKVDIE
VLSVLASLGA SVHKICTDIR LLANLKEMEE PFEKQQIGSS AMPYKRNPMR
SERCCSLARH LMTLVMDPLQ TASVQWFERT LDDSANRRIC LAEAFLTADT
ILNTLQNISE GLVVYPKVIE RRIRQELPFM ATENIIMAMV KAGGSRQDCH
EKIRVLSQQA ASVVKQEGGD NDLIERIQVD AYFSPIHSQL DHLLDPSSFT
GRASQQVQRF LEEEVYPLLK PYESVMKVKA ELCL
UCSC uc003ays.5; human. [P30566-1];

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anti-Adenylosuccinate lyase antibody

Category

Human ADSL ELISA Kit

Human ADSL ELISA Kit;Human ASL ELISA Kit;Human adenylosuccinase ELISA Kit;Human ASASE ELISA Kit;Human AMPS ELISA Kit;Human adenylosuccinate lyase ELISA Kit;