Human AK1 ELISA Kit

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  • Alternative name

    Human AK 1 ELISA Kit;Human ATP-AMP transphosphorylase 1 ELISA Kit;Human ATP:AMP phosphotransferase ELISA Kit;Human adenylate monophosphate kinase ELISA Kit;Human myokinase ELISA Kit;Human HTL-S-58j ELISA Kit;Human adenylate kinase 1 ELISA Kit;Human adenylate kinase isoenzyme 1 ELISA Kit;Human testis secretory sperm binding protein Li 58j ELISA Kit;

  • Catalog
    E000875
  • species
    Human
  • GeneAK1
  • Standard CurveHuman AK1 ELISA Kit
  • Other Species Mouse Ak1 ELISA KitBovine AK1 ELISA KitChicken AK1 ELISA KitPorcine AK1 ELISA KitRabbit AK1 ELISA KitRat Ak1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AK1. No significant cross-reactivity or interference between AK1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AK1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman AK1 ELISA Kit allows for the in vitro quantitative determination of AK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: AK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AK1 antibody and an AK1-HRP conjugate. The assay sample and buffer are incubated together with AK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK1 concentration since AK1 from samples and AK1-HRP conjugate compete for the anti-AK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK1 from the sample, fewer sites are left to bind AK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK1 concentration in each sample is interpolated from this standard curve.
  • Human Adenylate kinase isoenzyme 1 Protein information
  • Uniprot ID KAD1_HUMAN
  • Uniprot AC P00568; Q9BVK9; Q9UQC7;
  • UniGene Hs.175473;
  • GeneID 203
  • KEGG hsa:203;
  • Human Adenylate kinase isoenzyme 1 Protein SEQUENCE
  • SEQUENCE 194 AA; 21635 MW; 95EC5AAA92D1F00F CRC64;

    MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG

    SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ

    QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL

    ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK



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