Human AK1 ELISA Kit

Alternative name
Human AK 1 ELISA Kit;Human ATP-AMP transphosphorylase 1 ELISA Kit;Human ATP:AMP phosphotransferase ELISA Kit;Human adenylate monophosphate kinase ELISA Kit;Human myokinase ELISA Kit;Human HTL-S-58j ELISA Kit;Human adenylate kinase 1 ELISA Kit;Human adenylate kinase isoenzyme 1 ELISA Kit;Human testis secretory sperm binding protein Li 58j ELISA Kit;
Catalog
E000875
species
Human
GeneAK1
SpecificityThis assay has high sensitivity and excellent specificity for detection of AK1. No significant cross-reactivity or interference between AK1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AK1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman AK1 ELISA Kit allows for the in vitro quantitative determination of AK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: AK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AK1 antibody and an AK1-HRP conjugate. The assay sample and buffer are incubated together with AK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AK1 concentration since AK1 from samples and AK1-HRP conjugate compete for the anti-AK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AK1 from the sample, fewer sites are left to bind AK1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AK1 concentration in each sample is interpolated from this standard curve.
Human Adenylate kinase isoenzyme 1 Protein information
Uniprot ID KAD1_HUMAN
Uniprot AC P00568; Q9BVK9; Q9UQC7;
UniGene Hs.175473;
GeneID 203
KEGG hsa:203;
Human Adenylate kinase isoenzyme 1 Protein SEQUENCE
SEQUENCE 194 AA; 21635 MW; 95EC5AAA92D1F00F CRC64;
MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG
SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ
QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL
ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK

Human AK1 ELISA Kit

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