Human AK3 ELISA Kit

Alternative name
Human adenylate kinase 3 ELISA Kit;Human AK 3 ELISA Kit;Human adenylate kinase 3 alpha-like 1 ELISA Kit;Human AK3L1 ELISA Kit;Human AK6 ELISA Kit;Human AKL3L ELISA Kit;Human AKL3L1 ELISA Kit;Human FIX ELISA Kit;Human GTP:AMP phosphotransferase AK3, mitochondrial ELISA Kit;Human GTP:AMP phosphotransferase, mitochondrial ELISA Kit;Human adenylate kinase 6, adenylate kinase 3 like 1 ELISA Kit;
Catalog
E000870
species
Human
GeneAK3
SpecificityThis assay has high sensitivity and excellent specificity for detection of AK3. No significant cross-reactivity or interference between AK3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human AK3 is typically less than 0.146ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
Intended UseHuman AK3 ELISA Kit allows for the in vitro quantitative determination of AK3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AK3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to AK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human GTP:AMP phosphotransferase AK3, mitochondrial Protein information
Uniprot ID KAD3_HUMAN
Uniprot AC Q9UIJ7; B4DP58; D3DRI1; E7ET30; Q5VYW6; Q9H576; Q9HC01; Q9NPB4;
UniGene Hs.732022;
GeneID 50808
KEGG hsa:50808;
Human GTP:AMP phosphotransferase AK3, mitochondrial Protein SEQUENCE
SEQUENCE 227 AA; 25565 MW; 98A0EDF4FD9C9CEF CRC64;
MGASARLLRA VIMGAPGSGK GTVSSRITTH FELKHLSSGD LLRDNMLRGT
EIGVLAKAFI DQGKLIPDDV MTRLALHELK NLTQYSWLLD GFPRTLPQAE
ALDRAYQIDT VINLNVPFEV IKQRLTARWI HPASGRVYNI EFNPPKTVGI
DDLTGEPLIQ REDDKPETVI KRLKAYEDQT KPVLEYYQKK GVLETFSGTE
TNKIWPYVYA FLQTKVPQRS QKASVTP
UCSC uc003ziq.4; human. [Q9UIJ7-1];

Human AK3 ELISA Kit

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