Human CBX8 ELISA Kit

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  • Alternative name

    Human Polycomb 3 homolog ELISA Kit;Human PC3 ELISA Kit;Human hPc3 ELISA Kit;Human rectachrome 1 ELISA Kit;Human RC1 ELISA Kit;Human chromobox 8 ELISA Kit;Human chromobox protein homolog 8 ELISA Kit;Human Pc class 3 homolog ELISA Kit;Human chromobox homolog 8 (Pc class homolog, Drosophila) ELISA Kit;Human polycomb 3 ELISA Kit;

  • Catalog
    E008389
  • species
    Human
  • GeneCBX8
  • Standard CurveHuman CBX8 ELISA Kit
  • Other Species Mouse Cbx8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CBX8. No significant cross-reactivity or interference between CBX8 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CBX8 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CBX8 ELISA Kit allows for the in vitro quantitative determination of CBX8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Intended Uses: This CBX8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CBX8. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CBX8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CBX8 antibody and an CBX8-HRP conjugate. The assay sample and buffer are incubated together with CBX8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CBX8 concentration since CBX8 from samples and CBX8-HRP conjugate compete for the anti-CBX8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CBX8 from the sample, fewer sites are left to bind CBX8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CBX8 concentration in each sample is interpolated from this standard curve.
  • Human Chromobox protein homolog 8 Protein information
  • Uniprot ID CBX8_HUMAN
  • Uniprot AC Q9HC52; Q96H39; Q9NR07;
  • UniGene Hs.387258;
  • GeneID 57332
  • KEGG hsa:57332;
  • Human Chromobox protein homolog 8 Protein SEQUENCE
  • SEQUENCE 389 AA; 43396 MW; 651263C85B0ECD93 CRC64;

    MELSAVGERV FAAEALLKRR IRKGRMEYLV KWKGWSQKYS TWEPEENILD

    ARLLAAFEER EREMELYGPK KRGPKPKTFL LKAQAKAKAK TYEFRSDSAR

    GIRIPYPGRS PQDLASTSRA REGLRNMGLS PPASSTSTSS TCRAEAPRDR

    DRDRDRDRER DRERERERER ERERERERER GTSRVDDKPS SPGDSSKKRG

    PKPRKELPDP SQRPLGEPSA GLGEYLKGRK LDDTPSGAGK FPAGHSVIQL

    ARRQDSDLVQ CGVTSPSSAE ATGKLAVDTF PARVIKHRAA FLEAKGQGAL

    DPNGTRVRHG SGPPSSGGGL YRDMGAQGGR PSLIARIPVA RILGDPEEES

    WSPSLTNLEK VVVTDVTSNF LTVTIKESNT DQGFFKEKR

  • UCSC uc002jxd.3; human.;



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