Human CGb ELISA Kit

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  • Catalog
    E008355
  • species
    Human
  • GeneCGb
  • Standard CurveHuman CGb ELISA Kit
  • Other Species Mouse CGB ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Chorionic Gonadotropin Beta Polypeptide (CGb). No significant cross-reactivity or interference between Chorionic Gonadotropin Beta Polypeptide (CGb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 5.58ng/mL.
  • Intended UseHuman CGb ELISA Kit allows for the in vitro quantitative determination of CGb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Chorionic Gonadotropin Beta Polypeptide (CGb) and unlabeled Chorionic Gonadotropin Beta Polypeptide (CGb) (Standards or samples) with the pre-coated antibody specific to Chorionic Gonadotropin Beta Polypeptide (CGb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Chorionic Gonadotropin Beta Polypeptide (CGb) in the sample.



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