Human CHRD ELISA Kit

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  • Alternative name

    Human chordin ELISA Kit;

  • Catalog
    E008326
  • species
    Human
  • GeneCHRD
  • Other Species Mouse Chrd ELISA KitRat Chrd ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CHRD. No significant cross-reactivity or interference between human CHRD and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CHRD is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman CHRD ELISA Kit allows for the in vitro quantitative determination of CHRD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRD bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Chordin Protein information
  • Uniprot ID CHRD_HUMAN
  • Uniprot AC Q9P0Z3; Q9P0Z4; Q9P0Z5;
  • UniGene Hs.166186;
  • GeneID 8646
  • KEGG hsa:8646;
  • Human Chordin Protein SEQUENCE
  • SEQUENCE 955 AA; 102032 MW; 53F9D9F39A517686 CRC64;

    MPSLPAPPAP LLLLGLLLLG SRPARGAGPE PPVLPIRSEK EPLPVRGAAG

    CTFGGKVYAL DETWHPDLGE PFGVMRCVLC ACEAPQWGRR TRGPGRVSCK

    NIKPECPTPA CGQPRQLPGH CCQTCPQERS SSERQPSGLS FEYPRDPEHR

    SYSDRGEPGA EERARGDGHT DFVALLTGPR SQAVARARVS LLRSSLRFSI

    SYRRLDRPTR IRFSDSNGSV LFEHPAAPTQ DGLVCGVWRA VPRLSLRLLR

    AEQLHVALVT LTHPSGEVWG PLIRHRALAA ETFSAILTLE GPPQQGVGGI

    TLLTLSDTED SLHFLLLFRG LLEPRSGGLT QVPLRLQILH QGQLLRELQA

    NVSAQEPGFA EVLPNLTVQE MDWLVLGELQ MALEWAGRPG LRISGHIAAR

    KSCDVLQSVL CGADALIPVQ TGAAGSASLT LLGNGSLIYQ VQVVGTSSEV

    VAMTLETKPQ RRDQRTVLCH MAGLQPGGHT AVGICPGLGA RGAHMLLQNE

    LFLNVGTKDF PDGELRGHVA ALPYCGHSAR HDTLPVPLAG ALVLPPVKSQ

    AAGHAWLSLD THCHLHYEVL LAGLGGSEQG TVTAHLLGPP GTPGPRRLLK

    GFYGSEAQGV VKDLEPELLR HLAKGMASLM ITTKGSPRGE LRGQVHIANQ

    CEVGGLRLEA AGAEGVRALG APDTASAAPP VVPGLPALAP AKPGGPGRPR

    DPNTCFFEGQ QRPHGARWAP NYDPLCSLCT CQRRTVICDP VVCPPPSCPH

    PVQAPDQCCP VCPEKQDVRD LPGLPRSRDP GEGCYFDGDR SWRAAGTRWH

    PVVPPFGLIK CAVCTCKGGT GEVHCEKVQC PRLACAQPVR VNPTDCCKQC

    PVGSGAHPQL GDPMQADGPR GCRFAGQWFP ESQSWHPSVP PFGEMSCITC

    RCGAGVPHCE RDDCSLPLSC GSGKESRCCS RCTAHRRPAP ETRTDPELEK

    EAEGS

  • UCSC uc003fov.3; human. [Q9H2X0-1];




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