Human CHRNB3 ELISA Kit

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  • Alternative name

    Human cholinergic receptor nicotinic beta 3 subunit ELISA Kit;Human neuronal acetylcholine receptor subunit beta-3 ELISA Kit;Human acetylcholine receptor, neuronal nicotinic, beta-3 subunit ELISA Kit;Human acetylcholine receptor, nicotinic, beta 3 (neuronal) ELISA Kit;Human cholinergic receptor, nicotinic beta 3 ELISA Kit;Human cholinergic receptor, nicotinic, beta 3 (neuronal) ELISA Kit;Human cholinergic receptor, nicotinic, beta polypeptide 3 ELISA Kit;

  • Catalog
    E008294
  • species
    Human
  • GeneCHRNB3
  • Standard CurveHuman CHRNB3 ELISA Kit
  • Other Species Mouse Chrnb3 ELISA KitChicken CHRNB3 ELISA KitRat Chrnb3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CHRNb3. No significant cross-reactivity or interference between CHRNb3 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of CHRNb3 is typically less than 0.125ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding t
  • Intended UseHuman CHRNB3 ELISA Kit allows for the in vitro quantitative determination of CHRNB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHRNb3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CHRNb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHRNb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHRNb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neuronal acetylcholine receptor subunit beta-3 Protein information
  • Uniprot ID ACHB3_HUMAN
  • Uniprot AC Q05901; Q15827;
  • UniGene Hs.654576;
  • GeneID 1142
  • KEGG hsa:1142;
  • Human Neuronal acetylcholine receptor subunit beta-3 Protein SEQUENCE
  • SEQUENCE 458 AA; 52729 MW; D6E919E53CBD21F8 CRC64;

    MLPDFMLVLI VLGIPSSATT GFNSIAENED ALLRHLFQGY QKWVRPVLHS

    NDTIKVYFGL KISQLVDVDE KNQLMTTNVW LKQEWTDHKL RWNPDDYGGI

    HSIKVPSESL WLPDIVLFEN ADGRFEGSLM TKVIVKSNGT VVWTPPASYK

    SSCTMDVTFF PFDRQNCSMK FGSWTYDGTM VDLILINENV DRKDFFDNGE

    WEILNAKGMK GNRRDGVYSY PFITYSFVLR RLPLFYTLFL IIPCLGLSFL

    TVLVFYLPSD EGEKLSLSTS VLVSLTVFLL VIEEIIPSSS KVIPLIGEYL

    LFIMIFVTLS IIVTVFVINV HHRSSSTYHP MAPWVKRLFL QKLPKLLCMK

    DHVDRYSSPE KEESQPVVKG KVLEKKKQKQ LSDGEKVLVA FLEKAADSIR

    YISRHVKKEH FISQVVQDWK FVAQVLDRIF LWLFLIVSVT GSVLIFTPAL

    KMWLHSYH

  • UCSC uc003xpi.2; human.;


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