Human CHRNA5 ELISA Kit

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  • Alternative name

    Human NACHRA5 ELISA Kit;Human LNCR2 ELISA Kit;Human cholinergic receptor nicotinic alpha 5 subunit ELISA Kit;Human neuronal acetylcholine receptor subunit alpha-5 ELISA Kit;Human Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5 ELISA Kit;Human acetylcholine receptor, nicotinic, alpha 5 (neuronal) ELISA Kit;Human cholinergic receptor, nicotinic alpha 5 ELISA Kit;Human cholinergic receptor, nicotinic, alpha 5 (neuronal) ELISA Kit;Human neuronal nicotinic acetylcholine receptor, alpha5 subunit ELISA Kit;

  • Catalog
    E008286
  • species
    Human
  • GeneCHRNA5
  • Standard CurveHuman CHRNA5 ELISA Kit
  • Other Species Mouse Chrna5 ELISA KitBovine CHRNA5 ELISA KitChicken CHRNA5 ELISA KitRat Chrna5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5). No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.31ng/mL.
  • Intended UseHuman CHRNA5 ELISA Kit allows for the in vitro quantitative determination of CHRNA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neuronal acetylcholine receptor subunit alpha-5 Protein information
  • Uniprot ID ACHA5_HUMAN
  • Uniprot AC P30532; Q15824; Q99554;
  • UniGene Hs.1614; Hs.719767;
  • GeneID 1138
  • KEGG hsa:1138;
  • Human Neuronal acetylcholine receptor subunit alpha-5 Protein SEQUENCE
  • SEQUENCE 468 AA; 53054 MW; 7FE91A2E362289C0 CRC64;

    MAARGSGPRA LRLLLLVQLV AGRCGLAGAA GGAQRGLSEP SSIAKHEDSL

    LKDLFQDYER WVRPVEHLND KIKIKFGLAI SQLVDVDEKN QLMTTNVWLK

    QEWIDVKLRW NPDDYGGIKV IRVPSDSVWT PDIVLFDNAD GRFEGTSTKT

    VIRYNGTVTW TPPANYKSSC TIDVTFFPFD LQNCSMKFGS WTYDGSQVDI

    ILEDQDVDKR DFFDNGEWEI VSATGSKGNR TDSCCWYPYV TYSFVIKRLP

    LFYTLFLIIP CIGLSFLTVL VFYLPSNEGE KICLCTSVLV SLTVFLLVIE

    EIIPSSSKVI PLIGEYLVFT MIFVTLSIMV TVFAINIHHR SSSTHNAMAP

    LVRKIFLHTL PKLLCMRSHV DRYFTQKEET ESGSGPKSSR NTLEAALDSI

    RYITRHIMKE NDVREVVEDW KFIAQVLDRM FLWTFLFVSI VGSLGLFVPV

    IYKWANILIP VHIGNANK

  • UCSC uc002bdy.3; human.;


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