Human CHKA ELISA Kit

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  • Alternative name

    Human CK ELISA Kit;Human CHETK-alpha ELISA Kit;Human ethanolamine kinase ELISA Kit;Human EK ELISA Kit;Human CHK ELISA Kit;Human CKI ELISA Kit;Human choline kinase alpha ELISA Kit;

  • Catalog
    E008243
  • species
    Human
  • GeneCHKA
  • Other Species Mouse Chka ELISA KitRat Chka ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human CHKA. No significant cross-reactivity or interference between human CHKA and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human CHKA is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman CHKA ELISA Kit allows for the in vitro quantitative determination of CHKA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHKA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHKA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHKA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHKA bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Choline kinase alpha Protein information
  • Uniprot ID CHKA_HUMAN
  • Uniprot AC P35790; Q8NE29;
  • UniGene Hs.77221;
  • GeneID 1119
  • KEGG hsa:1119;
  • Human Choline kinase alpha Protein SEQUENCE
  • SEQUENCE 457 AA; 52249 MW; 65F177ABE1AA3A12 CRC64;

    MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG

    QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP

    GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA

    ILQMRSCNKE GSEQAQKENE FQGAEAMVLE SVMFAILAER SLGPKLYGIF

    PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM PFNKEPKWLF

    GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV

    VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW

    MYDYSYEKYP FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI

    IKEEMLLEVN RFALASHFLW GLWSIVQAKI SSIEFGYMDY AQARFDAYFH

    QKRKLGV

  • UCSC uc001onj.4; human. [P35790-1];


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