Human CHKA ELISA Kit

Alternative name
Human CK ELISA Kit;Human CHETK-alpha ELISA Kit;Human ethanolamine kinase ELISA Kit;Human EK ELISA Kit;Human CHK ELISA Kit;Human CKI ELISA Kit;Human choline kinase alpha ELISA Kit;
Catalog
E008243
species
Human
GeneCHKA
Other Species Mouse Chka ELISA Kit Rat Chka ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of human CHKA. No significant cross-reactivity or interference between human CHKA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CHKA is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman CHKA ELISA Kit allows for the in vitro quantitative determination of CHKA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHKA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHKA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHKA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHKA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Choline kinase alpha Protein information
Uniprot ID CHKA_HUMAN
Uniprot AC P35790; Q8NE29;
UniGene Hs.77221;
GeneID 1119
KEGG hsa:1119;
Human Choline kinase alpha Protein SEQUENCE
SEQUENCE 457 AA; 52249 MW; 65F177ABE1AA3A12 CRC64;
MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG
QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP
GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA
ILQMRSCNKE GSEQAQKENE FQGAEAMVLE SVMFAILAER SLGPKLYGIF
PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM PFNKEPKWLF
GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV
VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW
MYDYSYEKYP FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI
IKEEMLLEVN RFALASHFLW GLWSIVQAKI SSIEFGYMDY AQARFDAYFH
QKRKLGV
UCSC uc001onj.4; human. [P35790-1];

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anti-Choline kinase alpha antibody

Category

Human CHKA ELISA Kit

Human CK ELISA Kit;Human CHETK-alpha ELISA Kit;Human ethanolamine kinase ELISA Kit;Human EK ELISA Kit;Human CHK ELISA Kit;Human CKI ELISA Kit;Human choline kinase alpha ELISA Kit;