Human CH25H ELISA Kit

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  • Alternative name

    Human cholesterol 25-monooxygenase ELISA Kit;Human h25OH ELISA Kit;Human C25H ELISA Kit;Human cholesterol 25-hydroxylase ELISA Kit;

  • Catalog
    E008225
  • species
    Human
  • GeneCH25H
  • Standard CurveHuman CH25H ELISA Kit
  • Other Species Mouse Ch25h ELISA KitPorcine CH25H ELISA KitRat Ch25h ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholesterol-25-Hydroxylase (CH25H). No significant cross-reactivity or interference between Cholesterol-25-Hydroxylase (CH25H) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 49pg/mL.
  • Intended UseHuman CH25H ELISA Kit allows for the in vitro quantitative determination of CH25H , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesterol-25-Hydroxylase (CH25H). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesterol-25-Hydroxylase (CH25H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesterol-25-Hydroxylase (CH25H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesterol-25-Hydroxylase (CH25H) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cholesterol 25-hydroxylase Protein information
  • Uniprot ID CH25H_HUMAN
  • Uniprot AC O95992; B2RBY3;
  • UniGene Hs.47357;
  • GeneID 9023
  • KEGG hsa:9023;
  • Human Cholesterol 25-hydroxylase Protein SEQUENCE
  • SEQUENCE 272 AA; 31745 MW; 0DF46BC21802008B CRC64;

    MSCHNCSDPQ VLCSSGQLFL QPLWDHLRSW EALLQSPFFP VIFSITTYVG

    FCLPFVVLDI LCSWVPALRR YKIHPDFSPS AQQLLPCLGQ TLYQHVMFVF

    PVTLLHWARS PALLPHEAPE LLLLLHHILF CLLLFDMEFF VWHLLHHKVP

    WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT

    LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFNCNF

    APYFTHWDKI LGTLRTASVP AR

  • UCSC uc001kfz.4; human.;




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