Human CH25H ELISA Kit

Alternative name
Human cholesterol 25-monooxygenase ELISA Kit;Human h25OH ELISA Kit;Human C25H ELISA Kit;Human cholesterol 25-hydroxylase ELISA Kit;
Catalog
E008225
species
Human
GeneCH25H
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholesterol-25-Hydroxylase (CH25H). No significant cross-reactivity or interference between Cholesterol-25-Hydroxylase (CH25H) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 49pg/mL.
Intended UseHuman CH25H ELISA Kit allows for the in vitro quantitative determination of CH25H , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesterol-25-Hydroxylase (CH25H). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesterol-25-Hydroxylase (CH25H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesterol-25-Hydroxylase (CH25H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesterol-25-Hydroxylase (CH25H) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cholesterol 25-hydroxylase Protein information
Uniprot ID CH25H_HUMAN
Uniprot AC O95992; B2RBY3;
UniGene Hs.47357;
GeneID 9023
KEGG hsa:9023;
Human Cholesterol 25-hydroxylase Protein SEQUENCE
SEQUENCE 272 AA; 31745 MW; 0DF46BC21802008B CRC64;
MSCHNCSDPQ VLCSSGQLFL QPLWDHLRSW EALLQSPFFP VIFSITTYVG
FCLPFVVLDI LCSWVPALRR YKIHPDFSPS AQQLLPCLGQ TLYQHVMFVF
PVTLLHWARS PALLPHEAPE LLLLLHHILF CLLLFDMEFF VWHLLHHKVP
WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT
LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFNCNF
APYFTHWDKI LGTLRTASVP AR
UCSC uc001kfz.4; human.;

Human CH25H ELISA Kit

Human cholesterol 25-monooxygenase ELISA Kit;Human h25OH ELISA Kit;Human C25H ELISA Kit;Human cholesterol 25-hydroxylase ELISA Kit;

Contact Us