Human CYP11A1 ELISA Kit

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  • Alternative name

    Human CYPXIA1 ELISA Kit;Human Cholesterol desmolase ELISA Kit;Human cytochrome P450 11A1 ELISA Kit;Human cytochrome P450(scc) ELISA Kit;Human CYP11A ELISA Kit;Human P450SCC ELISA Kit;Human cytochrome P450 family 11 subfamily A member 1 ELISA Kit;Human cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit;Human cholesterol 20-22 desmolase ELISA Kit;Human cholesterol monooxygenase (side-chain cleaving) ELISA Kit;Human cytochrome P450 family 11 subfamily A polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily XIA (cholesterol side chain cleavage) ELISA Kit;Human cytochrome P450C11A1 ELISA Kit;Human steroid 20-22-lyase ELISA Kit;

  • Catalog
    E008223
  • species
    Human
  • GeneCYP11A1
  • Other Species Mouse Cyp11a1 ELISA KitBovine CYP11A1 ELISA KitGoat CYP11A1 ELISA KitHorse CYP11A1 ELISA KitMonkey CYP11A1 ELISA KitPorcine CYP11A1 ELISA KitRabbit CYP11A1 ELISA KitRat Cyp11a1 ELISA KitSheep CYP11A1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman CYP11A1 ELISA Kit allows for the in vitro quantitative determination of CYP11A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human CYP11A1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human CYP11A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYP11A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman CYP11A1can be calculated.
  • Human Cholesterol side-chain cleavage enzyme, mitochondrial Protein information
  • Uniprot ID CP11A_HUMAN
  • Uniprot AC P05108; A8K8D5; B3KPU8; G3XAD7; Q15081; Q16805; Q8N1A7;
  • UniGene Hs.303980;
  • GeneID 1583
  • KEGG hsa:1583;
  • Human Cholesterol side-chain cleavage enzyme, mitochondrial Protein SEQUENCE
  • SEQUENCE 521 AA; 60102 MW; AB0501E7A5665D8B CRC64;

    MLAKGLPPRS VLVKGCQTFL SAPREGLGRL RVPTGEGAGI STRSPRPFNE

    IPSPGDNGWL NLYHFWRETG THKVHLHHVQ NFQKYGPIYR EKLGNVESVY

    VIDPEDVALL FKSEGPNPER FLIPPWVAYH QYYQRPIGVL LKKSAAWKKD

    RVALNQEVMA PEATKNFLPL LDAVSRDFVS VLHRRIKKAG SGNYSGDISD

    DLFRFAFESI TNVIFGERQG MLEEVVNPEA QRFIDAIYQM FHTSVPMLNL

    PPDLFRLFRT KTWKDHVAAW DVIFSKADIY TQNFYWELRQ KGSVHHDYRG

    ILYRLLGDSK MSFEDIKANV TEMLAGGVDT TSMTLQWHLY EMARNLKVQD

    MLRAEVLAAR HQAQGDMATM LQLVPLLKAS IKETLRLHPI SVTLQRYLVN

    DLVLRDYMIP AKTLVQVAIY ALGREPTFFF DPENFDPTRW LSKDKNITYF

    RNLGFGWGVR QCLGRRIAEL EMTIFLINML ENFRVEIQHL SDVGTTFNLI

    LMPEKPISFT FWPFNQEATQ Q

  • UCSC uc002axs.3; human. [P05108-1];


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