Human CYP11A1 ELISA Kit

Alternative name
Human CYPXIA1 ELISA Kit;Human Cholesterol desmolase ELISA Kit;Human cytochrome P450 11A1 ELISA Kit;Human cytochrome P450(scc) ELISA Kit;Human CYP11A ELISA Kit;Human P450SCC ELISA Kit;Human cytochrome P450 family 11 subfamily A member 1 ELISA Kit;Human cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit;Human cholesterol 20-22 desmolase ELISA Kit;Human cholesterol monooxygenase (side-chain cleaving) ELISA Kit;Human cytochrome P450 family 11 subfamily A polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily XIA (cholesterol side chain cleavage) ELISA Kit;Human cytochrome P450C11A1 ELISA Kit;Human steroid 20-22-lyase ELISA Kit;
Catalog
E008223
species
Human
GeneCYP11A1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman CYP11A1 ELISA Kit allows for the in vitro quantitative determination of CYP11A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human CYP11A1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human CYP11A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYP11A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman CYP11A1can be calculated.
Human Cholesterol side-chain cleavage enzyme, mitochondrial Protein information
Uniprot ID CP11A_HUMAN
Uniprot AC P05108; A8K8D5; B3KPU8; G3XAD7; Q15081; Q16805; Q8N1A7;
UniGene Hs.303980;
GeneID 1583
KEGG hsa:1583;
Human Cholesterol side-chain cleavage enzyme, mitochondrial Protein SEQUENCE
SEQUENCE 521 AA; 60102 MW; AB0501E7A5665D8B CRC64;
MLAKGLPPRS VLVKGCQTFL SAPREGLGRL RVPTGEGAGI STRSPRPFNE
IPSPGDNGWL NLYHFWRETG THKVHLHHVQ NFQKYGPIYR EKLGNVESVY
VIDPEDVALL FKSEGPNPER FLIPPWVAYH QYYQRPIGVL LKKSAAWKKD
RVALNQEVMA PEATKNFLPL LDAVSRDFVS VLHRRIKKAG SGNYSGDISD
DLFRFAFESI TNVIFGERQG MLEEVVNPEA QRFIDAIYQM FHTSVPMLNL
PPDLFRLFRT KTWKDHVAAW DVIFSKADIY TQNFYWELRQ KGSVHHDYRG
ILYRLLGDSK MSFEDIKANV TEMLAGGVDT TSMTLQWHLY EMARNLKVQD
MLRAEVLAAR HQAQGDMATM LQLVPLLKAS IKETLRLHPI SVTLQRYLVN
DLVLRDYMIP AKTLVQVAIY ALGREPTFFF DPENFDPTRW LSKDKNITYF
RNLGFGWGVR QCLGRRIAEL EMTIFLINML ENFRVEIQHL SDVGTTFNLI
LMPEKPISFT FWPFNQEATQ Q
UCSC uc002axs.3; human. [P05108-1];

Category

Human CYP11A1 ELISA Kit