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Human CCKAR ELISA Kit

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  • Alternative name

    Human CCK-A receptor ELISA Kit;Human CCK-AR ELISA Kit;Human cholecystokinin-1 receptor ELISA Kit;Human CCK1-R ELISA Kit;Human CCKRA ELISA Kit;Human CCK-A ELISA Kit;Human CCK1R ELISA Kit;Human cholecystokinin A receptor ELISA Kit;Human cholecystokinin receptor type A ELISA Kit;Human cholecystokinin type-A receptor ELISA Kit;
  • Catalog
    E008206
  • species
    Human
  • GeneCCKAR
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholecystokinin A Receptor (CCKAR). No significant cross-reactivity or interference between Cholecystokinin A Receptor (CCKAR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.135ng/mL.
  • Intended UseHuman CCKAR ELISA Kit allows for the in vitro quantitative determination of CCKAR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor (CCKAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholecystokinin A Receptor (CCKAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholecystokinin A Receptor (CCKAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholecystokinin A Receptor (CCKAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cholecystokinin receptor type A Protein information
  • Uniprot ID CCKAR_HUMAN
  • Uniprot AC P32238; B2R9Z5;
  • UniGene Hs.129;
  • GeneID 886
  • KEGG hsa:886;
  • Human Cholecystokinin receptor type A Protein SEQUENCE

  • SEQUENCE 428 AA; 47841 MW; A6E8FABDA805E610 CRC64;

    MDVVDSLLVN GSNITPPCEL GLENETLFCL DQPRPSKEWQ PAVQILLYSL

    IFLLSVLGNT LVITVLIRNK RMRTVTNIFL LSLAVSDLML CLFCMPFNLI

    PNLLKDFIFG SAVCKTTTYF MGTSVSVSTF NLVAISLERY GAICKPLQSR

    VWQTKSHALK VIAATWCLSF TIMTPYPIYS NLVPFTKNNN QTANMCRFLL

    PNDVMQQSWH TFLLLILFLI PGIVMMVAYG LISLELYQGI KFEASQKKSA

    KERKPSTTSS GKYEDSDGCY LQKTRPPRKL ELRQLSTGSS SRANRIRSNS

    SAANLMAKKR VIRMLIVIVV LFFLCWMPIF SANAWRAYDT ASAERRLSGT

    PISFILLLSY TSSCVNPIIY CFMNKRFRLG FMATFPCCPN PGPPGARGEV

    GEEEEGGTTG ASLSRFSYSH MSASVPPQ

  • UCSC uc003gse.2; human.;



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