Human CCK8 ELISA Kit

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  • Catalog
    E008202
  • species
    Human
  • GeneCCK8
  • Standard CurveHuman CCK8 ELISA Kit
  • Other Species Mouse CCK8 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholecystokinin 8, Octapeptide (CCK8). No significant cross-reactivity or interference between Cholecystokinin 8, Octapeptide (CCK8) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.16pg/mL.
  • Intended UseHuman CCK8 ELISA Kit allows for the in vitro quantitative determination of CCK8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin 8, Octapeptide (CCK8) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin 8, Octapeptide (CCK8) and unlabeled Cholecystokinin 8, Octapeptide (CCK8) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin 8, Octapeptide (CCK8). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin 8, Octapeptide (CCK8) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin 8, Octapeptide (CCK8) in the sample.



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