Human AHCY ELISA Kit

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  • Alternative name

    Human adoHcyase ELISA Kit;Human S-adenosyl-L-homocysteine hydrolase ELISA Kit;Human SAHH ELISA Kit;Human adenosylhomocysteinase ELISA Kit;Human S-adenosylhomocysteine hydrolase ELISA Kit;

  • Catalog
    E000820
  • species
    Human
  • GeneAHCY
  • Standard CurveHuman AHCY ELISA Kit
  • Other Species Mouse Ahcy ELISA KitBovine AHCY ELISA KitMonkey AHCY ELISA KitPorcine AHCY ELISA KitRat Ahcy ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Adenosylhomocysteinase (AHCY). No significant cross-reactivity or interference between Adenosylhomocysteinase (AHCY) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman AHCY ELISA Kit allows for the in vitro quantitative determination of AHCY , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosylhomocysteinase (AHCY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosylhomocysteinase (AHCY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosylhomocysteinase (AHCY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosylhomocysteinase (AHCY) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Adenosylhomocysteinase Protein information
  • Uniprot ID SAHH_HUMAN
  • Uniprot AC P23526; A8K307; B3KUN3; E1P5P2; F5H737; Q96A36;
  • UniGene Hs.388004;
  • GeneID 191
  • KEGG hsa:191;
  • Human Adenosylhomocysteinase Protein SEQUENCE
  • SEQUENCE 432 AA; 47716 MW; 2833C025F969553E CRC64;

    MSDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI

    AGCLHMTVET AVLIETLVTL GAEVQWSSCN IFSTQDHAAA AIAKAGIPVY

    AWKGETDEEY LWCIEQTLYF KDGPLNMILD DGGDLTNLIH TKYPQLLPGI

    RGISEETTTG VHNLYKMMAN GILKVPAINV NDSVTKSKFD NLYGCRESLI

    DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI ITEIDPINAL

    QAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIG

    HFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLA EGRLVNLGCA

    MGHPSFVMSN SFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG

    KLNVKLTKLT EKQAQYLGMS CDGPFKPDHY RY

  • UCSC uc002xai.4; human. [P23526-1];


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