Human CCK18 ELISA Kit

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  • Catalog
    E008198
  • species
    Human
  • GeneCCK18
  • Standard CurveHuman CCK18 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholecystokinin 18 (CCK18). No significant cross-reactivity or interference between Cholecystokinin 18 (CCK18) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.36pg/mL.
  • Intended UseHuman CCK18 ELISA Kit allows for the in vitro quantitative determination of CCK18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin 18 (CCK18) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin 18 (CCK18) and unlabeled Cholecystokinin 18 (CCK18) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin 18 (CCK18). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin 18 (CCK18) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin 18 (CCK18) in the sample.



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