Human CLIC4 ELISA Kit

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  • Alternative name

    Human intracellular chloride ion channel protein p64H1 ELISA Kit;Human CLIC4L ELISA Kit;Human H1 ELISA Kit;Human MTCLIC ELISA Kit;Human huH1 ELISA Kit;Human p64H1 ELISA Kit;Human chloride intracellular channel 4 ELISA Kit;Human chloride intracellular channel protein 4 ELISA Kit;Human chloride intracellular channel 4 like ELISA Kit;

  • Catalog
    E008186
  • species
    Human
  • GeneCLIC4
  • Standard CurveHuman CLIC4 ELISA Kit
  • Other Species Mouse Clic4 ELISA KitBovine CLIC4 ELISA KitRat Clic4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CLIC4. No significant cross-reactivity or interference between CLIC4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLIC4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CLIC4 ELISA Kit allows for the in vitro quantitative determination of CLIC4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: CLIC4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLIC4 antibody and an CLIC4-HRP conjugate. The assay sample and buffer are incubated together with CLIC4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLIC4 concentration since CLIC4 from samples and CLIC4-HRP conjugate compete for the anti-CLIC4 antibody binding site. Since the number of sites is limited, as more sites are occupied by CLIC4 from the sample, fewer sites are left to bind CLIC4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLIC4 concentration in each sample is interpolated from this standard curve.
  • Human Chloride intracellular channel protein 4 Protein information
  • Uniprot ID CLIC4_HUMAN
  • Uniprot AC Q9Y696; Q9UFW9; Q9UQJ6;
  • UniGene Hs.440544;
  • GeneID 25932
  • KEGG hsa:25932;
  • Human Chloride intracellular channel protein 4 Protein SEQUENCE
  • SEQUENCE 253 AA; 28772 MW; 3DD21A33C66626A5 CRC64;

    MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV

    VFSVTTVDLK RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC

    PPKYLKLSPK HPESNTAGMD IFAKFSAYIK NSRPEANEAL ERGLLKTLQK

    LDEYLNSPLP DEIDENSMED IKFSTRKFLD GNEMTLADCN LLPKLHIVKV

    VAKKYRNFDI PKEMTGIWRY LTNAYSRDEF TNTCPSDKEV EIAYSDVAKR

    LTK

  • UCSC uc001bjo.3; human.;


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