Human CHI3L1 ELISA Kit

Alternative name
Human 39 kDa synovial protein ELISA Kit;Human cartilage glycoprotein 39 ELISA Kit;Human CGP-39 ELISA Kit;Human GP-39 ELISA Kit;Human hCGP-39 ELISA Kit;Human YKL-40 ELISA Kit;Human ASRT7 ELISA Kit;Human GP39 ELISA Kit;Human HC-gp39 ELISA Kit;Human HCGP-3P ELISA Kit;Human YKL40 ELISA Kit;Human YYL-40 ELISA Kit;Human chitinase 3 like 1 ELISA Kit;Human chitinase-3-like protein 1 ELISA Kit;Human chitinase 3-like 1 (cartilage glycoprotein-39) ELISA Kit;
Catalog
E008116
species
Human
GeneCHI3L1
Standard Curve
Other Species Human YKL-40/CHI3L1 ELISA Kit Mouse Chi3l1 ELISA Kit Mouse YKL-40/CHI3L1 ELISA Kit Bovine CHI3L1 ELISA Kit Goat CHI3L1 ELISA Kit Porcine CHI3L1 ELISA Kit Rat Chi3l1 ELISA Kit Sheep CHI3L1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHI3L1. No significant cross-reactivity or interference between CHI3L1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHI3L1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CHI3L1 ELISA Kit allows for the in vitro quantitative determination of CHI3L1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CHI3L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHI3L1 antibody and an CHI3L1-HRP conjugate. The assay sample and buffer are incubated together with CHI3L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHI3L1 concentration since CHI3L1 from samples and CHI3L1-HRP conjugate compete for the anti-CHI3L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHI3L1 from the sample, fewer sites are left to bind CHI3L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHI3L1 concentration in each sample is interpolated from this standard curve.
Human Chitinase-3-like protein 1 Protein information
Uniprot ID CH3L1_HUMAN
Uniprot AC P36222; B2R7B0; P30923; Q8IVA4; Q96HI7;
UniGene Hs.382202; Hs.733359;
GeneID 1116
KEGG hsa:1116;
Human Chitinase-3-like protein 1 Protein SEQUENCE
SEQUENCE 383 AA; 42625 MW; 6C9EF133BDC7C2D1 CRC64;
MGVKASQTGF VVLVLLQCCS AYKLVCYYTS WSQYREGDGS CFPDALDRFL
CTHIIYSFAN ISNDHIDTWE WNDVTLYGML NTLKNRNPNL KTLLSVGGWN
FGSQRFSKIA SNTQSRRTFI KSVPPFLRTH GFDGLDLAWL YPGRRDKQHF
TTLIKEMKAE FIKEAQPGKK QLLLSAALSA GKVTIDSSYD IAKISQHLDF
ISIMTYDFHG AWRGTTGHHS PLFRGQEDAS PDRFSNTDYA VGYMLRLGAP
ASKLVMGIPT FGRSFTLASS ETGVGAPISG PGIPGRFTKE AGTLAYYEIC
DFLRGATVHR ILGQQVPYAT KGNQWVGYDD QESVKSKVQY LKDRQLAGAM
VWALDLDDFQ GSFCGQDLRF PLTNAIKDAL AAT
UCSC uc001gzi.3; human.;

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Human CHI3L1 ELISA Kit