Human CHI3L1 ELISA Kit

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  • Alternative name

    Human 39 kDa synovial protein ELISA Kit;Human cartilage glycoprotein 39 ELISA Kit;Human CGP-39 ELISA Kit;Human GP-39 ELISA Kit;Human hCGP-39 ELISA Kit;Human YKL-40 ELISA Kit;Human ASRT7 ELISA Kit;Human GP39 ELISA Kit;Human HC-gp39 ELISA Kit;Human HCGP-3P ELISA Kit;Human YKL40 ELISA Kit;Human YYL-40 ELISA Kit;Human chitinase 3 like 1 ELISA Kit;Human chitinase-3-like protein 1 ELISA Kit;Human chitinase 3-like 1 (cartilage glycoprotein-39) ELISA Kit;

  • Catalog
    E008116
  • species
    Human
  • GeneCHI3L1
  • Standard CurveHuman CHI3L1 ELISA Kit
  • Other Species Human YKL-40/CHI3L1 ELISA KitMouse Chi3l1 ELISA KitMouse YKL-40/CHI3L1 ELISA KitBovine CHI3L1 ELISA KitGoat CHI3L1 ELISA KitPorcine CHI3L1 ELISA KitRat Chi3l1 ELISA KitSheep CHI3L1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CHI3L1. No significant cross-reactivity or interference between CHI3L1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHI3L1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CHI3L1 ELISA Kit allows for the in vitro quantitative determination of CHI3L1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: CHI3L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CHI3L1 antibody and an CHI3L1-HRP conjugate. The assay sample and buffer are incubated together with CHI3L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHI3L1 concentration since CHI3L1 from samples and CHI3L1-HRP conjugate compete for the anti-CHI3L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHI3L1 from the sample, fewer sites are left to bind CHI3L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHI3L1 concentration in each sample is interpolated from this standard curve.
  • Human Chitinase-3-like protein 1 Protein information
  • Uniprot ID CH3L1_HUMAN
  • Uniprot AC P36222; B2R7B0; P30923; Q8IVA4; Q96HI7;
  • UniGene Hs.382202; Hs.733359;
  • GeneID 1116
  • KEGG hsa:1116;
  • Human Chitinase-3-like protein 1 Protein SEQUENCE
  • SEQUENCE 383 AA; 42625 MW; 6C9EF133BDC7C2D1 CRC64;

    MGVKASQTGF VVLVLLQCCS AYKLVCYYTS WSQYREGDGS CFPDALDRFL

    CTHIIYSFAN ISNDHIDTWE WNDVTLYGML NTLKNRNPNL KTLLSVGGWN

    FGSQRFSKIA SNTQSRRTFI KSVPPFLRTH GFDGLDLAWL YPGRRDKQHF

    TTLIKEMKAE FIKEAQPGKK QLLLSAALSA GKVTIDSSYD IAKISQHLDF

    ISIMTYDFHG AWRGTTGHHS PLFRGQEDAS PDRFSNTDYA VGYMLRLGAP

    ASKLVMGIPT FGRSFTLASS ETGVGAPISG PGIPGRFTKE AGTLAYYEIC

    DFLRGATVHR ILGQQVPYAT KGNQWVGYDD QESVKSKVQY LKDRQLAGAM

    VWALDLDDFQ GSFCGQDLRF PLTNAIKDAL AAT

  • UCSC uc001gzi.3; human.;




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