Human CHIT1 ELISA Kit

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  • Alternative name

    Human Chitinase-1 ELISA Kit;Human CHI3 ELISA Kit;Human CHIT ELISA Kit;Human CHITD ELISA Kit;Human chitinase 1 ELISA Kit;Human chitotriosidase-1 ELISA Kit;Human chitinase 1 (chitotriosidase) ELISA Kit;Human plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase ELISA Kit;

  • Catalog
    E008113
  • species
    Human
  • GeneCHIT1
  • Standard CurveHuman CHIT1 ELISA Kit
  • Other Species Mouse Chit1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Chitinase 1 (CHIT1). No significant cross-reactivity or interference between Chitinase 1 (CHIT1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.129ng/mL.
  • Intended UseHuman CHIT1 ELISA Kit allows for the in vitro quantitative determination of CHIT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase 1 (CHIT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chitinase 1 (CHIT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chitinase 1 (CHIT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chitinase 1 (CHIT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Chitotriosidase-1 Protein information
  • Uniprot ID CHIT1_HUMAN
  • Uniprot AC Q9UDJ8;
  • UniGene Hs.201688;
  • GeneID 1118
  • KEGG hsa:1118;
  • Human Chitotriosidase-1 Protein SEQUENCE
  • SEQUENCE 466 AA; 51681 MW; B4312D1E885E386D CRC64;

    MVRSVAWAGF MVLLMIPWGS AAKLVCYFTN WAQYRQGEAR FLPKDLDPSL

    CTHLIYAFAG MTNHQLSTTE WNDETLYQEF NGLKKMNPKL KTLLAIGGWN

    FGTQKFTDMV ATANNRQTFV NSAIRFLRKY SFDGLDLDWE YPGSQGSPAV

    DKERFTTLVQ DLANAFQQEA QTSGKERLLL SAAVPAGQTY VDAGYEVDKI

    AQNLDFVNLM AYDFHGSWEK VTGHNSPLYK RQEESGAAAS LNVDAAVQQW

    LQKGTPASKL ILGMPTYGRS FTLASSSDTR VGAPATGSGT PGPFTKEGGM

    LAYYEVCSWK GATKQRIQDQ KVPYIFRDNQ WVGFDDVESF KTKVSYLKQK

    GLGGAMVWAL DLDDFAGFSC NQGRYPLIQT LRQELSLPYL PSGTPELEVP

    KPGQPSEPEH GPSPGQDTFC QGKADGLYPN PRERSSFYSC AAGRLFQQSC

    PTGLVFSNSC KCCTWN

  • UCSC uc001gzn.3; human. [Q13231-1];


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