Human ADK ELISA Kit

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  • Alternative name

    Human AK ELISA Kit;Human adenosine 5'-phosphotransferase ELISA Kit;Human adenosine kinase ELISA Kit;Human testicular tissue protein Li 14 ELISA Kit;

  • Catalog
    E000803
  • species
    Human
  • GeneADK
  • Standard CurveHuman ADK ELISA Kit
  • Other Species Mouse Adk ELISA KitHamster ADK ELISA KitRat Adk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Adenosine Kinase (ADK). No significant cross-reactivity or interference between Adenosine Kinase (ADK) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.59ng/mL.
  • Intended UseHuman ADK ELISA Kit allows for the in vitro quantitative determination of ADK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine Kinase (ADK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine Kinase (ADK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine Kinase (ADK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine Kinase (ADK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Adenosine kinase Protein information
  • Uniprot ID ADK_HUMAN
  • Uniprot AC Q9BTN2;
  • UniGene Hs.656586;
  • GeneID 132
  • KEGG hsa:132;
  • Human Adenosine kinase Protein SEQUENCE
  • SEQUENCE 362 AA; 40545 MW; 48AA4925865BFE70 CRC64;

    MAAAEEEPKP KKLKVEAPQA LRENILFGMG NPLLDISAVV DKDFLDKYSL

    KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA

    ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS

    LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA

    HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE

    QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA

    VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR

    TGCTFPEKPD FH

  • UCSC uc001jwi.4; human. [P55263-1];


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