Human ADK ELISA Kit

Alternative name
Human AK ELISA Kit;Human adenosine 5'-phosphotransferase ELISA Kit;Human adenosine kinase ELISA Kit;Human testicular tissue protein Li 14 ELISA Kit;
Catalog
E000803
species
Human
GeneADK
Standard Curve
Other Species Mouse Adk ELISA Kit Hamster ADK ELISA Kit Rat Adk ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Adenosine Kinase (ADK). No significant cross-reactivity or interference between Adenosine Kinase (ADK) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.59ng/mL.
Intended UseHuman ADK ELISA Kit allows for the in vitro quantitative determination of ADK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine Kinase (ADK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenosine Kinase (ADK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine Kinase (ADK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine Kinase (ADK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Adenosine kinase Protein information
Uniprot ID ADK_HUMAN
Uniprot AC Q9BTN2;
UniGene Hs.656586;
GeneID 132
KEGG hsa:132;
Human Adenosine kinase Protein SEQUENCE
SEQUENCE 362 AA; 40545 MW; 48AA4925865BFE70 CRC64;
MAAAEEEPKP KKLKVEAPQA LRENILFGMG NPLLDISAVV DKDFLDKYSL
KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA
ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS
LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA
HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE
QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA
VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR
TGCTFPEKPD FH
UCSC uc001jwi.4; human. [P55263-1];

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Category

Human ADK ELISA Kit

Human AK ELISA Kit;Human adenosine 5'-phosphotransferase ELISA Kit;Human adenosine kinase ELISA Kit;Human testicular tissue protein Li 14 ELISA Kit;