Human CCR7 ELISA Kit

Alternative name
Human C-C CKR-7 ELISA Kit;Human CC-CKR-7 ELISA Kit;Human CCR-7 ELISA Kit;Human BLR2 ELISA Kit;Human CDw197 ELISA Kit;Human Epstein-Barr virus-induced G-protein coupled receptor 1 ELISA Kit;Human EBI1 ELISA Kit;Human EBV-induced G-protein coupled receptor 1 ELISA Kit;Human MIP-3 beta receptor ELISA Kit;Human CMKBR7 ELISA Kit;Human EVI1 ELISA Kit;Human CD197 ELISA Kit;Human C-C motif chemokine receptor 7 ELISA Kit;Human C-C chemokine receptor type 7 ELISA Kit;Human Bukitt's lymphoma receptor 2 ELISA Kit;Human CC chemokine receptor 7 ELISA Kit;Human EBV-induced G protein-coupled receptor 1 ELISA Kit;Human Epstein-Barr virus induced gene 1 ELISA Kit;Human chemokine (C-C motif) receptor 7 ELISA Kit;Human lymphocyte-specific G protein-coupled peptide receptor ELISA Kit;
Catalog
E008013
species
Human
GeneCCR7
Standard Curve
Other Species Mouse Ccr7 ELISA Kit Bovine CCR7 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CCL-7. No significant cross-reactivity or interference between CCL-7 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CCL-7 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman CCR7 ELISA Kit allows for the in vitro quantitative determination of CCR7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This CCL-7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CCL-7. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CCL-7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL-7 antibody and an CCL-7-HRP conjugate. The assay sample and buffer are incubated together with CCL-7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-7 concentration since CCL-7 from samples and CCL-7-HRP conjugate compete for the anti-CCL-7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-7 from the sample, fewer sites are left to bind CCL-7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-7 concentration in each sample is interpolated from this standard curve.
Human C-C chemokine receptor type 7 Protein information
Uniprot ID CCR7_HUMAN
Uniprot AC P32248;
UniGene Hs.370036;
GeneID 1236
KEGG hsa:1236;
Human C-C chemokine receptor type 7 Protein SEQUENCE
SEQUENCE 378 AA; 42874 MW; D4CB4213841A1BD4 CRC64;
MDLGKPMKSV LVVALLVIFQ VCLCQDEVTD DYIGDNTTVD YTLFESLCSK
KDVRNFKAWF LPIMYSIICF VGLLGNGLVV LTYIYFKRLK TMTDTYLLNL
AVADILFLLT LPFWAYSAAK SWVFGVHFCK LIFAIYKMSF FSGMLLLLCI
SIDRYVAIVQ AVSAHRHRAR VLLISKLSCV GIWILATVLS IPELLYSDLQ
RSSSEQAMRC SLITEHVEAF ITIQVAQMVI GFLVPLLAMS FCYLVIIRTL
LQARNFERNK AIKVIIAVVV VFIVFQLPYN GVVLAQTVAN FNITSSTCEL
SKQLNIAYDV TYSLACVRCC VNPFLYAFIG VKFRNDLFKL FKDLGCLSQE
QLRQWSSCRH IRRSSMSVEA ETTTTFSP
UCSC uc002huw.4; human.;

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Human CCR7 ELISA Kit