Human CCL 8 ELISA Kit

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  • Alternative name

    C-C motif chemokine 8 ELISA KIT; HC14 ELISA KIT; Monocyte chemoattractant protein 2 ELISA KIT; Monocyte chemotactic protein 2 ELISA KIT; MCP-2 ELISA KIT; Small-inducible cytokine A8Cleaved into the following chain:MCP-2(6-76)CCL8 ELISA KIT; MCP2 ELISA KIT; SCYA10 ELISA KIT; SCYA8 ELISA KIT; MCP-2 ELISA KIT

  • Catalog
    E008012
  • species
    Human
  • GeneCCL 8
  • Other Species Human CCL-8 ELISA KitMouse CCL-8 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman CCL 8 ELISA Kit allows for the in vitro quantitative determination of CCL 8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the Assay: CCL8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CCL8 antibody and an CCL8-HRP conjugate. The assay sample and buffer are incubated together with CCL8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL8 concentration since CCL8 from samples and CCL8-HRP conjugate compete for the anti-CCL8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL8 from the sample, fewer sites are left to bind CCL8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL8 concentration in each sample is interpolated from this standard curve.




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