Human CHAT ELISA Kit

Alternative name
Human CHOACTASE ELISA Kit;Human ChAT ELISA Kit;Human choline acetylase ELISA Kit;Human CMS1A ELISA Kit;Human CMS1A2 ELISA Kit;Human CMS6 ELISA Kit;Human choline O-acetyltransferase ELISA Kit;Human acetyl CoA:choline O-acetyltransferase ELISA Kit;
Catalog
E007933
species
Human
GeneCHAT
Standard Curve
Other Species Mouse Chat ELISA Kit Chicken CHAT ELISA Kit Porcine CHAT ELISA Kit Rat Chat ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHAT . No significant cross-reactivity or interference between CHAT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 9.375pg/ml
Intended UseHuman CHAT ELISA Kit allows for the in vitro quantitative determination of CHAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CHATantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CHAT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CHAT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CHAT can be calculated.
Human Choline O-acetyltransferase Protein information
Uniprot ID CLAT_HUMAN
Uniprot AC P28329; A2BDF4; A2BDF5; Q16488; Q9BQ23; Q9BQ35; Q9BQE1;
UniGene Hs.302002;
GeneID 1103
KEGG hsa:1103;
Human Choline O-acetyltransferase Protein SEQUENCE
SEQUENCE 748 AA; 82536 MW; A902364081915391 CRC64;
MGLRTAKKRG LGGGGKWKRE EGGGTRGRRE VRPACFLQSG GRGDPGDVGG
PAGNPGCSPH PRAATRPPPL PAHTPAHTPE WCGAASAEAA EPRRAGPHLC
IPAPGLTKTP ILEKVPRKMA AKTPSSEESG LPKLPVPPLQ QTLATYLQCM
RHLVSEEQFR KSQAIVQQFG APGGLGETLQ QKLLERQEKT ANWVSEYWLN
DMYLNNRLAL PVNSSPAVIF ARQHFPGTDD QLRFAASLIS GVLSYKALLD
SHSIPTDCAK GQLSGQPLCM KQYYGLFSSY RLPGHTQDTL VAQNSSIMPE
PEHVIVACCN QFFVLDVVIN FRRLSEGDLF TQLRKIVKMA SNEDERLPPI
GLLTSDGRSE WAEARTVLVK DSTNRDSLDM IERCICLVCL DAPGGVELSD
THRALQLLHG GGYSKNGANR WYDKSLQFVV GRDGTCGVVC EHSPFDGIVL
VQCTEHLLKH VTQSSRKLIR ADSVSELPAP RRLRWKCSPE IQGHLASSAE
KLQRIVKNLD FIVYKFDNYG KTFIKKQKCS PDAFIQVALQ LAFYRLHRRL
VPTYESASIR RFQEGRVDNI RSATPEALAF VRAVTDHKAA VPASEKLLLL
KDAIRAQTAY TVMAITGMAI DNHLLALREL ARAMCKELPE MFMDETYLMS
NRFVLSTSQV PTTTEMFCCY GPVVPNGYGA CYNPQPETIL FCISSFHSCK
ETSSSKFAKA VEESLIDMRD LCSLLPPTES KPLATKEKAT RPSQGHQP
UCSC uc001jhv.1; human. [P28329-1];

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Category

Human CHAT ELISA Kit

Human CHOACTASE ELISA Kit;Human ChAT ELISA Kit;Human choline acetylase ELISA Kit;Human CMS1A ELISA Kit;Human CMS1A2 ELISA Kit;Human CMS6 ELISA Kit;Human choline O-acetyltransferase ELISA Kit;Human acetyl CoA:choline O-acetyltransferase ELISA Kit;