Human CFI ELISA Kit

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  • Alternative name

    Human C3B/C4B inactivator ELISA Kit;Human IF ELISA Kit;Human AHUS3 ELISA Kit;Human ARMD13 ELISA Kit;Human C3BINA ELISA Kit;Human C3b-INA ELISA Kit;Human FI ELISA Kit;Human KAF ELISA Kit;Human complement factor I ELISA Kit;Human C3b-inactivator ELISA Kit;Human Konglutinogen-activating factor ELISA Kit;Human complement component I ELISA Kit;Human complement control protein factor I ELISA Kit;Human complement factor I heavy chain ELISA Kit;Human light chain of factor I ELISA Kit;

  • Catalog
    E007888
  • species
    Human
  • GeneCFI
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CFI. No significant cross-reactivity or interference between Human CFI and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human CFI and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.406ng/mL
  • Intended UseHuman CFI ELISA Kit allows for the in vitro quantitative determination of CFI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFI in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human CFI and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human CFI. You can calculate the concentration of Human CFI in the samples by comparing the OD of the samples to the standard curve.
  • Human Complement factor I light chain Protein information
  • Uniprot ID CFAI_HUMAN
  • Uniprot AC P05156; O60442;
  • UniGene Hs.312485;
  • GeneID 3426
  • KEGG hsa:3426;
  • Human Complement factor I light chain Protein SEQUENCE
  • SEQUENCE 583 AA; 65750 MW; F06070EFE6B572A1 CRC64;

    MKLLHVFLLF LCFHLRFCKV TYTSQEDLVE KKCLAKKYTH LSCDKVFCQP

    WQRCIEGTCV CKLPYQCPKN GTAVCATNRR SFPTYCQQKS LECLHPGTKF

    LNNGTCTAEG KFSVSLKHGN TDSEGIVEVK LVDQDKTMFI CKSSWSMREA

    NVACLDLGFQ QGADTQRRFK LSDLSINSTE CLHVHCRGLE TSLAECTFTK

    RRTMGYQDFA DVVCYTQKAD SPMDDFFQCV NGKYISQMKA CDGINDCGDQ

    SDELCCKACQ GKGFHCKSGV CIPSQYQCNG EVDCITGEDE VGCAGFASVT

    QEETEILTAD MDAERRRIKS LLPKLSCGVK NRMHIRRKRI VGGKRAQLGD

    LPWQVAIKDA SGITCGGIYI GGCWILTAAH CLRASKTHRY QIWTTVVDWI

    HPDLKRIVIE YVDRIIFHEN YNAGTYQNDI ALIEMKKDGN KKDCELPRSI

    PACVPWSPYL FQPNDTCIVS GWGREKDNER VFSLQWGEVK LISNCSKFYG

    NRFYEKEMEC AGTYDGSIDA CKGDSGGPLV CMDANNVTYV WGVVSWGENC

    GKPEFPGVYT KVANYFDWIS YHVGRPFISQ YNV

  • UCSC uc003hzr.5; human.;



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